2 Commits d63638d00a ... c48df7e917

Auteur SHA1 Bericht Datum
  Zhang Xudong c48df7e917 add example 2 jaren geleden
  Zhang Xudong d97453fec2 add example 2 jaren geleden
3 gewijzigde bestanden met toevoegingen van 114 en 253845 verwijderingen
  1. 32 1
      README.md
  2. 3 84
      example_data/out.emapper.annotations
  3. 79 253760
      example_data/proteins.fa

+ 32 - 1
README.md

@@ -1 +1,32 @@
-# emcp
+# 安装
+
+## 安装依赖
+1. R        
+2. R 包:argparser, tidyverse, formattable, AnnotationForge, seqinr, clusterProfiler       
+
+## 安装 emcp
+
+```
+git clone http://git.genek.cn:3333/zhxd2/emcp.git
+```
+
+# 使用
+
+## 准备输入文件
+
+输入文件为蛋白序列, 参考 example_data 目录下的 proteins.fa        
+
+## 第一步:在线注释
+登录 http://eggnog-mapper.embl.de/ 上传蛋白序列, 在线进行蛋白注释.          
+完成后下载 out.emapper.annotations     
+
+
+## 第二步:运行 emapperx.R
+
+```
+Rscript ../emapperx.R out.emapper.annotations proteins.fa
+```
+
+这一步两个功能:    
+1. 对 emapper 注释结果进行统计绘图      
+2. 构建 OrgDB 用于富集分析等     

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example_data/out.emapper.annotations


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example_data/proteins.fa


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