'textfield', '#title' => t('GFF3 File'), '#description' => t('Please enter the full system path for the GFF file, or a path within the Drupal installation (e.g. /sites/default/files/xyz.gff). The path must be accessible to the server on which this Drupal instance is running.'), '#required' => TRUE, '#weight' => 1 ); // get the list of organisms $sql = "SELECT * FROM {organism} ORDER BY genus, species"; $previous_db = tripal_db_set_active('chado'); // use chado database $org_rset = db_query($sql); tripal_db_set_active($previous_db); // now use drupal database $organisms = array(); $organisms[''] = ''; while ($organism = db_fetch_object($org_rset)) { $organisms[$organism->organism_id] = "$organism->genus $organism->species ($organism->common_name)"; } $form['organism_id'] = array( '#title' => t('Organism'), '#type' => t('select'), '#description' => t("Choose the organism to which these sequences are associated"), '#required' => TRUE, '#options' => $organisms, ); $form['import_options'] = array( '#type' => 'fieldset', '#title' => t('Import Options'), '#weight' => 6, '#collapsed' => TRUE ); $form['import_options']['add_only']= array( '#type' => 'checkbox', '#title' => t('Import only new features'), '#required' => FALSE, '#description' => t('The job will skip features in the GFF file that already exist in the database and import only new features.'), '#weight' => 2 ); $form['import_options']['update']= array( '#type' => 'checkbox', '#title' => t('Import all and update'), '#required' => FALSE, '#default_value' => 'checked', '#description' => t('Existing features will be updated and new features will be added. Attributes for a feature that are not present in the GFF but which are present in the database will not be altered.'), '#weight' => 3 ); $form['import_options']['refresh']= array( '#type' => 'checkbox', '#title' => t('Import all and replace'), '#required' => FALSE, '#description' => t('Existing features will be updated and feature properties not present in the GFF file will be removed.'), '#weight' => 4 ); $form['import_options']['remove']= array( '#type' => 'checkbox', '#title' => t('Delete features'), '#required' => FALSE, '#description' => t('Features present in the GFF file that exist in the database will be removed rather than imported'), '#weight' => 5 ); $form['analysis'] = array( '#type' => 'fieldset', '#title' => t('Analysis Used to Derive Features'), '#weight' => 6, '#collapsed' => TRUE ); $form['analysis']['desc'] = array( '#type' => 'markup', '#value' => t("Why specify an analysis for a data load? All data comes from some place, even if downloaded from Genbank. By specifying analysis details for all data uploads, it allows an end user to reproduce the data set, but at least indicates the source of the data."), ); // get the list of analyses $sql = "SELECT * FROM {analysis} ORDER BY name"; $previous_db = tripal_db_set_active('chado'); // use chado database $org_rset = db_query($sql); tripal_db_set_active($previous_db); // now use drupal database $analyses = array(); $analyses[''] = ''; while ($analysis = db_fetch_object($org_rset)) { $analyses[$analysis->analysis_id] = "$analysis->name ($analysis->program $analysis->programversion, $analysis->sourcename)"; } $form['analysis']['analysis_id'] = array( '#title' => t('Analysis'), '#type' => t('select'), '#description' => t("Choose the analysis to which these features are associated"), '#required' => TRUE, '#options' => $analyses, ); $form['button'] = array( '#type' => 'submit', '#value' => t('Import GFF3 file'), '#weight' => 10, ); return $form; } /** * * * @ingroup gff3_loader */ function tripal_feature_gff3_load_form_validate($form, &$form_state) { $gff_file = $form_state['values']['gff_file']; $organism_id = $form_state['values']['organism_id']; $add_only = $form_state['values']['add_only']; $update = $form_state['values']['update']; $refresh = $form_state['values']['refresh']; $remove = $form_state['values']['remove']; // check to see if the file is located local to Drupal $gff_file = trim($gff_file); $dfile = $_SERVER['DOCUMENT_ROOT'] . base_path() . $gff_file; if (!file_exists($dfile)) { // if not local to Drupal, the file must be someplace else, just use // the full path provided $dfile = $gff_file; } if (!file_exists($dfile)) { form_set_error('gff_file', t("Cannot find the file on the system. Check that the file exists or that the web server has permissions to read the file.")); } // @coder-ignore: there are no functions being called here // @todo: break each line of this conditional into separate variables to make more readable if (($add_only AND ($update OR $refresh OR $remove)) OR ($update AND ($add_only OR $refresh OR $remove)) OR ($refresh AND ($update OR $add_only OR $remove)) OR ($remove AND ($update OR $refresh OR $add_only))) { form_set_error('add_only', t("Please select only one checkbox from the import options section")); } } /** * * @ingroup gff3_loader */ function tripal_feature_gff3_load_form_submit($form, &$form_state) { global $user; $gff_file = $form_state['values']['gff_file']; $organism_id = $form_state['values']['organism_id']; $add_only = $form_state['values']['add_only']; $update = $form_state['values']['update']; $refresh = $form_state['values']['refresh']; $remove = $form_state['values']['remove']; $analysis_id = $form_state['values']['analysis_id']; $args = array($gff_file, $organism_id, $analysis_id, $add_only, $update, $refresh, $remove); $type = ''; if ($add_only) { $type = 'import only new features'; } if ($update) { $type = 'import all and update'; } if ($refresh) { $type = 'import all and replace'; } if ($remove) { $type = 'delete features'; } $fname = preg_replace("/.*\/(.*)/", "$1", $gff_file); tripal_add_job("$type GFF3 file: $fname", 'tripal_feature', 'tripal_feature_load_gff3', $args, $user->uid); return ''; } /** * * * @ingroup gff3_loader */ function tripal_feature_load_gff3($gff_file, $organism_id, $analysis_id, $add_only =0, $update = 0, $refresh = 0, $remove = 0, $job = NULL) { // make sure our temporary table exists $ret = array(); if (!db_table_exists('tripal_gff_temp')) { $schema = tripal_feature_get_custom_tables('tripal_gff_temp'); $success = tripal_core_create_custom_table($ret, 'tripal_gff_temp', $schema['tripal_gff_temp']); if(!$success) { watchdog('T_gff3_loader', "Cannot creat temporary loading table", array(), WATCHDOG_ERROR); return; } } // empty the temp table $sql = "DELETE FROM tripal_gff_temp"; chado_query($sql); // begin the transaction $connection = tripal_db_start_transaction(); // if we cannot get a connection then let the user know the loading will be slow if (!$connection) { print "A persistant connection was not obtained. Loading will be slow\n"; } else { print "\nNOTE: Loading of this GFF file is performed using a database transaction. If the load " . "fails or is terminated prematurely then the entire set of insertions/updates is rolled back " . "and will not be found in the database\n\n"; } // check to see if the file is located local to Drupal $dfile = $_SERVER['DOCUMENT_ROOT'] . base_path() . $gff_file; if (!file_exists($dfile)) { // if not local to Drupal, the file must be someplace else, just use // the full path provided $dfile = $gff_file; } if (!file_exists($dfile)) { watchdog('T_gff3_loader',"Cannot find the file: %dfile", array('%dfile' => $dfile), WATCHDOG_ERROR); return 0; } //$previous_db = tripal_db_set_active('chado'); print "Opening $gff_file\n"; //$lines = file($dfile,FILE_SKIP_EMPTY_LINES); $fh = fopen($dfile, 'r'); if (!$fh) { watchdog('T_gff3_loader',"cannot open file: %dfile", array('%dfile' => $dfile), WATCHDOG_ERROR); return 0; } $filesize = filesize($dfile); // get the controlled vocaubulary that we'll be using. The // default is the 'sequence' ontology // @coder-ignore: non-drupal schema thus table prefixing does not apply $sql = "SELECT * FROM cv WHERE name = '%s'"; $cv = db_fetch_object(db_query($sql, 'sequence')); if (!$cv) { watchdog('T_gff3_loader',"Cannot find the 'sequence' ontology", array(), WATCHDOG_ERROR); return ''; } // get the organism for which this GFF3 file belongs // @coder-ignore: non-drupal schema thus table prefixing does not apply $sql = "SELECT * FROM organism WHERE organism_id = %d"; $organism = db_fetch_object(db_query($sql, $organism_id)); $interval = intval($filesize * 0.0001); if ($interval == 0) { $interval = 1; } $in_fasta = 0; $line_num = 0; $num_read = 0; $intv_read = 0; // iterate through each line of the GFF file print "Parsing Line $line_num (0.00%). memory: " . memory_get_usage() . "\r"; while ($line = fgets($fh)) { $line_num++; $num_read += drupal_strlen($line); $intv_read += $num_read; // update the job status every 1% features if ($job and $intv_read >= $interval) { $intv_read = 0; $percent = sprintf("%.2f", ($num_read / $filesize) * 100); print "Parsing Line $line_num (" . $percent . "%). memory: " . memory_get_usage() . ".\r"; tripal_job_set_progress($job, intval(($num_read / $filesize) * 100)); } // check to see if we have FASTA section, if so then set the variable // to start parsing if (preg_match('/^##FASTA/i', $line)) { $in_fasta = 1; break; } // skip comments if (preg_match('/^#/', $line)) { continue; } // skip empty lines if (preg_match('/^\s*$/', $line)) { continue; } // TODO: handle FASTA section // get the columns $cols = explode("\t", $line); if (sizeof($cols) != 9) { watchdog('T_gff3_loader','improper number of columns on line %line_num', array('%line_num' => $line_num), WATCHDOG_ERROR); return ''; } // get the column values $landmark = $cols[0]; $source = $cols[1]; $type = $cols[2]; $start = $cols[3]; $end = $cols[4]; $score = $cols[5]; $strand = $cols[6]; $phase = $cols[7]; $attrs = explode(";", $cols[8]); // split by a semicolon // ready the start and stop for chado. Chado expects these positions // to be zero-based, so we substract 1 from the fmin $fmin = $start - 1; $fmax = $end; if ($end < $start) { $fmin = $end - 1; $fmax = $start; } // format the strand for chado if (strcmp($strand, '.') == 0) { $strand = 0; } elseif (strcmp($strand, '+') == 0) { $strand = 1; } elseif (strcmp($strand, '-') == 0) { $strand = -1; } if (strcmp($phase, '.') == 0) { $phase = ''; } // get the type record if (!tripal_core_is_sql_prepared('sel_cvterm_cvid_cvtname_synonym')) { $psql = "PREPARE sel_cvterm_cvid_cvtname_synonym (int, text, text) AS SELECT CVT.cvterm_id, CVT.cv_id, CVT.name, CVT.definition, CVT.dbxref_id, CVT.is_obsolete, CVT.is_relationshiptype FROM {cvterm} CVT INNER JOIN {cv} CV on CVT.cv_id = CV.cv_id LEFT JOIN {cvtermsynonym} CVTS on CVTS.cvterm_id = CVT.cvterm_id WHERE CV.cv_id = $1 and (CVT.name = $2 or CVTS.synonym = $3)"; $status = chado_query($psql); if (!$status) { watchdog('T_gff3_loader','cannot prepare statement \'sel_cvterm_cvid_cvtname_synonym\' for ontology term %line_num', array('%line_num' => $line_num), WATCHDOG_ERROR); return ''; } } $result = chado_query("EXECUTE sel_cvterm_cvid_cvtname_synonym (%d, '%s', '%s')", $cv->cv_id, $type, $type); $cvterm = db_fetch_object($result); if (!$cvterm) { watchdog('T_gff3_loader','cannot find ontology term \'%type\' on line %line_num', array('%type' => $type, '%line_num' => $line_num), WATCHDOG_ERROR); return ''; } // break apart each of the attributes $tags = array(); $attr_name = ''; $attr_uniquename = ''; $attr_residue_info = ''; $attr_locgroup = 0; $attr_fmin_partial = 'f'; $attr_fmax_partial = 'f'; $attr_is_obsolete = 'f'; $attr_is_analysis = 'f'; $attr_others = ''; $residues = ''; foreach ($attrs as $attr) { $attr = rtrim($attr); $attr = ltrim($attr); if (strcmp($attr, '')==0) { continue; } if (!preg_match('/^[^\=]+\=.+$/', $attr)) { watchdog('T_gff3_loader','Attribute is not correctly formatted on line %line_num: %attr', array('%line_num' => $line_num, '%attr' => $attr), WATCHDOG_ERROR); return ''; } // break apart each tag $tag = preg_split("/=/", $attr, 2); // split by equals sign // multiple instances of an attribute are separated by commas $tag_name = $tag[0]; if (!array_key_exists($tag_name, $tags)) { $tags[$tag_name] = array(); } $tags[$tag_name] = array_merge($tags[$tag_name], explode(",", $tag[1])); // split by comma // replace the URL escape codes for each tag for ($i = 0; $i < count($tags[$tag_name]); $i++) { $tags[$tag_name][$i] = urldecode($tags[$tag_name][$i]); } // get the name and ID tags if (strcmp($tag_name, 'ID') == 0) { $attr_uniquename = $tag[1]; } elseif (strcmp($tag_name, 'Name') == 0) { $attr_name = $tag[1]; } // get the list of non-reserved attributes elseif (strcmp($tag_name, 'Alias') !=0 and strcmp($tag_name, 'Parent') !=0 and strcmp($tag_name, 'Target') !=0 and strcmp($tag_name, 'Gap') !=0 and strcmp($tag_name, 'Derives_from') !=0 and strcmp($tag_name, 'Note') !=0 and strcmp($tag_name, 'Dbxref') !=0 and strcmp($tag_name, 'Ontology_term') !=0 and strcmp($tag_name, 'Is_circular') !=0) { foreach ($tags[$tag_name] as $value){ $attr_others[$tag_name][] = $value; } } } // if neither name nor uniquename are provided then generate one if (!$attr_uniquename and !$attr_name) { if (array_key_exists('Parent', $tags)) { $attr_uniquename = $tags['Parent'][0] . "-$type-$landmark:$fmin..$fmax"; } else { watchdog('T_gff3_loader','Cannot generate a uniquename for feature on line %line_num', array('%line_num' => $line_num), WATCHDOG_ERROR); exit; } $attr_name = $attr_uniquename; } // if a name is not specified then use the unique name if (strcmp($attr_name, '')==0) { $attr_name = $attr_uniquename; } // if an ID attribute is not specified then use the attribute name and // hope for the best if (!$attr_uniquename) { $attr_uniquename = $attr_name; } // make sure the landmark sequence exists in the database. We don't // know the type of the landmark so we'll hope that it's unique across // all types. If not we'll error out. This test is only necessary if // if the landmark and the uniquename are different. If they are the same // then this is the information for the landmark if (strcmp($landmark, $attr_uniquename) != 0 ) { $select = array( 'organism_id' => $organism_id, 'uniquename' => $landmark, ); $columns = array('count(*) as num_landmarks'); $options = array('statement_name' => 'sel_feature_organismid_uniquename'); $count = tripal_core_chado_select('feature', $columns, $select, $options); if (!$count or $count[0]->num_landmarks == 0) { watchdog('T_gff3_loader',"The landmark '%landmark' cannot be found for this organism. ". "Please add the landmark and then retry the import of this GFF3 ". "file", array('%landmark' => $landmark), WATCHDOG_ERROR); return ''; } if ($count[0]->num_landmarks > 1) { watchdog('T_gff3_loader',"The landmark '%landmark' is not unique for this organism. ". "The features cannot be associated", array('%landmark' => $landmark), WATCHDOG_ERROR); return ''; } } // if the option is to remove or refresh then we want to remove // the feature from the database. if ($remove or $refresh) { $sql = "DELETE FROM {feature} WHERE organism_id = %d and uniquename = '%s' and type_id = %d"; $match = array( 'organism_id' => $organism->organism_id, 'uniquename' => $attr_uniquename, 'type_id' => $cvterm->cvterm_id ); $result = tripal_core_chado_delete('feature',$match); if (!$result) { watchdog('T_gff3_loader',"cannot delete feature %attr_uniquename", array('%attr_uniquename' => $attr_uniquename), WATCHDOG_ERROR); } $feature = 0; unset($result); } // add or update the feature and all properties if ($update or $refresh or $add_only) { // add/update the feature $feature = tripal_feature_load_gff3_feature($organism, $analysis_id, $cvterm, $attr_uniquename, $attr_name, $residues, $attr_is_analysis, $attr_is_obsolete, $add_only, $score); if ($feature) { // add a record for this feature to the tripal_gff_temp table for // later lookup $values = array( 'feature_id' => $feature->feature_id, 'organism_id' => $feature->organism_id, 'type_name' => $type, 'uniquename' => $feature->uniquename ); $options = array('statement_name' => 'ins_tripalgfftemp'); $result = tripal_core_chado_insert('tripal_gff_temp', $values, $options); if (!$result) { watchdog('T_gff3_loader',"Cound not save record in temporary table, Cannot continue.", array(), WATCHDOG_ERROR); return; } // add/update the featureloc if the landmark and the ID are not the same // if they are the same then this entry in the GFF is probably a landmark identifier if (strcmp($landmark, $attr_uniquename) !=0 ) { tripal_feature_load_gff3_featureloc($feature, $organism, $landmark, $fmin, $fmax, $strand, $phase, $attr_fmin_partial, $attr_fmax_partial, $attr_residue_info, $attr_locgroup); } // add any aliases for this feature if (array_key_exists('Alias', $tags)) { tripal_feature_load_gff3_alias($feature, $tags['Alias']); } // add any dbxrefs for this feature if (array_key_exists('Dbxref', $tags)) { tripal_feature_load_gff3_dbxref($feature, $tags['Dbxref']); } // add any ontology terms for this feature if (array_key_exists('Ontology_term', $tags)) { tripal_feature_load_gff3_ontology($feature, $tags['Ontology_term']); } // add parent relationships if (array_key_exists('Parent', $tags)) { tripal_feature_load_gff3_parents($feature, $cvterm, $tags['Parent'], $organism_id, $fmin); } // add target relationships if (array_key_exists('Target', $tags)) { preg_match('/^(.*)\s+\d+\s+\d+\s+(\+|\-)*$/', $tags['Target'][0], $matches); $target_feature = $matches[1]; $start = $matches[2]; $end = $matches[3]; if ($matches[4]) { $target_strand = $matches[4]; } else { $target_strand = '.'; } $target_fmin = $start - 1; $target_fmax = $end; if ($end < $start) { $target_fmin = $end - 1; $target_fmax = $start; } #print "Target: $target_feature, $target_fmin-$target_fmax $target_dir\n"; tripal_feature_load_gff3_featureloc($feature, $organism, $target_feature, $target_fmin, $target_fmax, $target_strand, $phase, $attr_fmin_partial, $attr_fmax_partial, $attr_residue_info, $attr_locgroup); } // add gap information. This goes in simply as a property if (array_key_exists('Gap', $tags)) { foreach ($tags['Gap'] as $value) { tripal_feature_load_gff3_property($feature, 'Gap', $value); } } // add notes. This goes in simply as a property if (array_key_exists('Note', $tags)) { foreach ($tags['Note'] as $value) { tripal_feature_load_gff3_property($feature, 'Note', $value); } } // add the Derives_from relationship (e.g. polycistronic genes). if (array_key_exists('Derives_from', $tags)) { tripal_feature_load_gff3_derives_from($feature, $tags['Derives_from'][0], $organism); } // add in the GFF3_source dbxref so that GBrowse can find the feature using the source column $source_ref = array('GFF_source:' . $source); tripal_feature_load_gff3_dbxref($feature, $source_ref); // add any additional attributes if ($attr_others) { foreach ($attr_others as $tag_name => $values) { foreach ($values as $value){ tripal_feature_load_gff3_property($feature, $tag_name, $value); } } } } } } print "\nSetting ranks of children...\n"; // get features in a parent relationship $sql = "SELECT DISTINCT F.feature_id, F.organism_id, F.type_id, F.uniquename, FL.strand FROM tripal_gff_temp TGT INNER JOIN feature F on TGT.feature_id = F.feature_id INNER JOIN feature_relationship FR ON FR.object_id = TGT.feature_id INNER JOIN cvterm CVT on CVT.cvterm_id = FR.type_id INNER JOIN featureloc FL on FL.feature_id = F.feature_id WHERE CVT.name = 'part_of'"; $parents = chado_query($sql); // build and prepare the SQL for selecting the children relationship $sql = "SELECT FR.feature_relationship_id, FL.fmin, FR.rank FROM feature_relationship FR INNER JOIN featureloc FL on FL.feature_id = FR.subject_id"; if (!$connection) { $sql .= "WHERE FR.object_id = %d ". "ORDER BY FL.fmin ASC "; } else { $sql = "PREPARE sel_gffchildren (int) AS " . $sql . " WHERE FR.object_id = \$1 ORDER BY FL.fmin ASC"; } if (!tripal_core_is_sql_prepared('sel_gffchildren')) { $success = chado_query($sql); if (!$success) { watchdog("T_gff3_loader", "Cannot prepare statement 'sel_gffchildren' and cannot set children ranks.", array(), WATCHDOG_WARNING); return 0; } } // now set the rank of any parent/child relationships. The order is based // on the fmin. The start rank is 1. This allows features with other // relationships to be '0' (the default), and doesn't interfer with the // ordering defined here. while ($parent = db_fetch_object($parents)) { // get the children if ($connection) { $result = chado_query('EXECUTE sel_gffchildren (%d)', $parent->feature_id); } else { $result = chado_query($sql, $parent->feature_id); } // build an array of the children $children = array(); while ($child = db_fetch_object($result)) { $children[] = $child; } // sort the children come in order of their fmin position // but if the parent is on the reverse strand we need to // reverse the order of the children. if ($parent->strand == -1) { arsort($children); } // first set the ranks to a negative number so that we don't // get a duplicate error message when we try to change any of them $rank = -1; foreach ($children as $child) { $match = array('feature_relationship_id' => $child->feature_relationship_id); $options = array('statement_name' => 'upd_featurerelationship_rank'); $values = array('rank' => $rank); tripal_core_chado_update('feature_relationship', $match, $values, $options); $rank--; } // now set the rank correctly $rank = 0; foreach ($children as $child) { $match = array('feature_relationship_id' => $child->feature_relationship_id); $options = array('statement_name' => 'upd_featurerelationship_rank'); $values = array('rank' => $rank); //print "Was: " . $child->rank . " now $rank ($parent->strand)\n" ; tripal_core_chado_update('feature_relationship', $match, $values, $options); $rank++; } } // commit the transaction tripal_db_commit_transaction(); print "Done\n"; return 1; } /** * * * @ingroup gff3_loader */ function tripal_feature_load_gff3_derives_from($feature, $subject, $organism) { // get the subject type $values = array( 'organism_id' => $organism->organism_id, 'uniquename' => $subject, ); $options = array('statement_name' => 'sel_tripalgfftemp_orun'); $result = tripal_core_chado_select('tripal_gff_temp', array('type_name'), $values, $options); if (count($result) == 0) { watchdog("T_gff3_loader", "Cannot find subject type for feature in 'derives_from' relationship: %subject", array('%subject' => $subject), WATCHDOG_WARNING); return ''; } $subject_type = $result[0]->type_name; // get the subject feature $match = array( 'organism_id' => $organism->organism_id, 'uniquename' => $subject, 'type_id' => array( 'name' => $subject_type, 'cv_id' => array( 'name' => 'sequence' ), ), ); $options = array('statement_name' => 'sel_feature_orunty'); $sfeature = tripal_core_chado_select('feature', array('*'), $match, $options); if (count($sfeature)==0) { watchdog('T_gff3_loader',"Could not add 'Derives_from' relationship ". "for %uniquename and %subject. Subject feature, '%subject', ". "cannot be found", array('%uniquename' => $feature->uniquename, '%subject' => $subject), WATCHDOG_ERROR); return; } // now check to see if the relationship already exists $values = array( 'object_id' => $sfeature[0]->feature_id, 'subject_id' => $feature->feature_id, 'type_id' => array( 'cv_id' => array( 'name' => 'relationship' ), 'name' => 'derives_from', ), 'rank' => 0 ); $options = array('statement_name' => 'sel_featurerelationship_objectid_subjectid_typeid_rank'); $rel = tripal_core_chado_select('feature_relationship', array('*'), $values, $options); if (count($rel) > 0) { return; } // finally insert the relationship if it doesn't exist $options = array('statement_name' => 'ins_featurerelationship_objectid_subjectid_typeid_rank'); $ret = tripal_core_chado_insert('feature_relationship', $values, $options); if (!$ret) { watchdog("T_gff3_loader", "Could not add 'Derives_from' relationship for $feature->uniquename and $subject", array(), WATCHDOG_WARNING); } } /** * * * @ingroup gff3_loader */ function tripal_feature_load_gff3_parents($feature, $cvterm, $parents, $organism_id, $fmin) { $uname = $feature->uniquename; $type = $cvterm->name; $rel_type = 'part_of'; // prepare these SQL statements that will be used repeatedly. if (!tripal_core_is_sql_prepared('sel_cvterm_cvname_cvtname_synonym')) { $psql = "PREPARE sel_cvterm_cvname_cvtname_synonym (text, text, text) AS SELECT CVT.cvterm_id FROM cvterm CVT INNER JOIN cv CV on CVT.cv_id = CV.cv_id LEFT JOIN cvtermsynonym CVTS on CVTS.cvterm_id = CVT.cvterm_id WHERE cv.name = $1 and (CVT.name = $2 or CVTS.synonym = $3)"; $status = chado_query($psql); if (!$status) { watchdog("T_gff3_loader", "Cannot prepare statement 'sel_cvterm_cvname_cvtname_synonym' for ontology term", array(), WATCHDOG_WARNING); return ''; } } // iterate through the parents in the list foreach ($parents as $parent) { // get the parent cvterm $values = array( 'organism_id' => $organism_id, 'uniquename' => $parent, ); $options = array('statement_name' => 'sel_tripalgfftemp_orun'); $result = tripal_core_chado_select('tripal_gff_temp', array('type_name'), $values, $options); if (count($result) == 0) { watchdog("T_gff3_loader", "Cannot find parent type: %parent", array('%parent' => $parent), WATCHDOG_WARNING); return ''; } $parent_type = $result[0]->type_name; // try to find the parent $parentcvterm = db_fetch_object(chado_query("EXECUTE sel_cvterm_cvname_cvtname_synonym ('%s', '%s', '%s')", 'sequence', $parent_type, $parent_type)); $relcvterm = db_fetch_object(chado_query("EXECUTE sel_cvterm_cvname_cvtname_synonym ('%s', '%s', '%s')", 'relationship', $rel_type, $rel_type)); $values = array( 'organism_id' => $organism_id, 'uniquename' => $parent, 'type_id' => $parentcvterm->cvterm_id, ); $options = array('statement_name' => 'sel_feature_orunty'); $result = tripal_core_chado_select('feature', array('*'), $values, $options); $parent_feature = $result[0]; // if the parent exists then add the relationship otherwise print error and skip if ($parent_feature) { // check to see if the relationship already exists $values = array( 'object_id' => $parent_feature->feature_id, 'subject_id' => $feature->feature_id, 'type_id' => $relcvterm->cvterm_id, ); $options = array('statement_name' => 'sel_featurerelationship_objectid_subjectid_typeid'); $rel = tripal_core_chado_select('feature_relationship', array('*'), $values, $options); if (count($rel) > 0) { } else { // the relationship doesn't already exist, so add it. $values = array( 'subject_id' => $feature->feature_id, 'object_id' => $parent_feature->feature_id, 'type_id' => $relcvterm->cvterm_id, ); $options = array('statement_name' => 'ins_featurerelationship_subjectid_objectid_typeid'); $result = tripal_core_chado_insert('feature_relationship', $values, $options); if (!$result) { watchdog("T_gff3_loader", "Failed to insert feature relationship '$uname' ($type) $rel_type '$parent' ($parent_type)", array(), WATCHDOG_WARNING); } } } else { watchdog("T_gff3_loader", "Cannot establish relationship '$uname' ($type) $rel_type '$parent' ($parent_type): Cannot find the parent", array(), WATCHDOG_WARNING); } } } /** * * * @ingroup gff3_loader */ function tripal_feature_load_gff3_dbxref($feature, $dbxrefs) { // iterate through each of the dbxrefs foreach ($dbxrefs as $dbxref) { // get the database name from the reference. If it doesn't exist then create one. $ref = explode(":", $dbxref); $dbname = $ref[0]; $accession = $ref[1]; // first look for the database name if it doesn't exist then create one. // first check for the fully qualified URI (e.g. DB:. If that // can't be found then look for the name as is. If it still can't be found // the create the database $options = array('statement_name' => 'sel_db_name'); $db = tripal_core_chado_select('db', array('db_id'), array('name' => "DB:$dbname"), $options); if (count($db) == 0) { $db = tripal_core_chado_select('db', array('db_id'), array('name' => "$dbname"), $options); } if (count($db) == 0) { $options = array('statement_name' => 'ins_db_name'); $ret = tripal_core_chado_insert('db', array('name' => $dbname, 'description' => 'Added automatically by the GFF loader'), $options); if ($ret) { $options = array('statement_name' => 'sel_db_name'); $db = tripal_core_chado_select('db', array('db_id'), array('name' => "$dbname"), $options); } else { watchdog("T_gff3_loader", "Cannot find or add the database $dbname", array(), WATCHDOG_WARNING); return 0; } } $db = $db[0]; // now check to see if the accession exists $options = array('statement_name' => 'sel_dbxref_accession_dbid'); $dbxref = tripal_core_chado_select('dbxref', array('dbxref_id'), array( 'accession' => $accession, 'db_id' => $db->db_id), $options); // if the accession doesn't exist then we want to add it if (sizeof($dbxref) == 0) { $options = array('statement_name' => 'ins_dbxref_dbid_accession_version'); $ret = tripal_core_chado_insert('dbxref', array('db_id' => $db->db_id, 'accession' => $accession, 'version' => ''), $options); $options = array('statement_name' => 'sel_dbxref_accession_dbid'); $dbxref = tripal_core_chado_select('dbxref', array('dbxref_id'), array( 'accession' => $accession, 'db_id' => $db->db_id), $options); } $dbxref = $dbxref[0]; // check to see if this feature dbxref already exists $options = array('statement_name' => 'sel_featuredbxref_dbxrefid_featureid'); $fdbx = tripal_core_chado_select('feature_dbxref', array('feature_dbxref_id'), array('dbxref_id' => $dbxref->dbxref_id, 'feature_id' => $feature->feature_id), $options); // now associate this feature with the database reference if it doesn't // already exist if (sizeof($fdbx)==0) { $options = array('statement_name' => 'ins_featuredbxref_dbxrefid_featureid'); $ret = tripal_core_chado_insert('feature_dbxref', array( 'dbxref_id' => $dbxref->dbxref_id, 'feature_id' => $feature->feature_id), $options); if ($ret) { } else { watchdog("T_gff3_loader", "Failed to insert Dbxref: $dbname:$accession", array(), WATCHDOG_WARNING); return 0; } } else { } } return 1; } /** * * * @ingroup gff3_loader */ function tripal_feature_load_gff3_ontology($feature, $dbxrefs) { // iterate through each of the dbxrefs foreach ($dbxrefs as $dbxref) { // get the database name from the reference. If it doesn't exist then create one. $ref = explode(":", $dbxref); $dbname = $ref[0]; $accession = $ref[1]; // first look for the database name $options = array('statement_name' => 'sel_db_name'); $db = tripal_core_chado_select('db', array('db_id'), array('name' => "DB:$dbname"), $options); if (sizeof($db) == 0) { $db = tripal_core_chado_select('db', array('db_id'), array('name' => "$dbname"), $options); } if (sizeof($db) == 0) { watchdog("T_gff3_loader", "Database, $dbname is missing for reference: $dbname:$accession", array(), WATCHDOG_WARNING); return 0; } $db = $db[0]; // now check to see if the accession exists $options = array('statement_name' => 'sel_dbxref_accession_dbid'); $dbxref = tripal_core_chado_select('dbxref', array('dbxref_id'), array( 'accession' => $accession, 'db_id' => $db->db_id), $options); if (sizeof($dbxref) == 0) { watchdog("T_gff3_loader", "Accession, $accession is missing for reference: $dbname:$accession", array(), WATCHDOG_WARNING); return 0; } $dbxref = $dbxref[0]; // now check to see if the cvterm exists $options = array('statement_name' => 'sel_cvterm_dbxrefid'); $cvterm = tripal_core_chado_select('cvterm', array('cvterm_id'), array( 'dbxref_id' => $dbxref->dbxref_id), $options); // if it doesn't exist in the cvterm table, look for an alternate id if (sizeof($cvterm) == 0) { $options = array('statement_name' => 'sel_cvtermdbxref_dbxrefid'); $cvterm = tripal_core_chado_select('cvterm_dbxref', array('cvterm_id'), array( 'dbxref_id' => $dbxref->dbxref_id), $options); } if (sizeof($cvterm) == 0) { watchdog("T_gff3_loader", "CV Term is missing for reference: $dbname:$accession", array(), WATCHDOG_WARNING); return 0; } $cvterm = $cvterm[0]; // check to see if this feature cvterm already exists $options = array('statement_name' => 'sel_featurecvterm_cvtermid_featureid'); $fcvt = tripal_core_chado_select('feature_cvterm', array('feature_cvterm_id'), array('cvterm_id' => $cvterm->cvterm_id, 'feature_id' => $feature->feature_id), $options); // now associate this feature with the cvterm if it doesn't already exist if (sizeof($fcvt)==0) { $values = array( 'cvterm_id' => $cvterm->cvterm_id, 'feature_id' => $feature->feature_id, 'pub_id' => array( 'uniquename' => 'null', ), ); $options = array('statement_name' => 'ins_featurecvterm_cvtermid_featureid_pubid'); $ret = tripal_core_chado_insert('feature_cvterm', $values, $options); if ($ret) { } else { watchdog("T_gff3_loader", "Failed to insert ontology term: $dbname:$accession", array(), WATCHDOG_WARNING); return 0; } } } return 1; } /** * * * @ingroup gff3_loader */ function tripal_feature_load_gff3_alias($feature, $aliases) { // make sure we have a 'synonym_type' vocabulary $select = array('name' => 'synonym_type'); $options = array('statement_name' => 'sel_cv_name'); $results = tripal_core_chado_select('cv', array('*'), $selct, $options); if (count($results) == 0) { // insert the 'synonym_type' vocabulary $values = array( 'name' => 'synonym_type', 'definition' => 'vocabulary for synonym types', ); $options = array('statement_name' => 'ins_cv_name_definition'); $result = tripal_core_chado_insert('cv', $values, $options); if (!result) { watchdog("T_gff3_loader", "Failed to add the synonyms type vocabulary", array(), WATCHDOG_WARNING); return 0; } // now that we've added the cv we need to get the record $options = array('statement_name' => 'sel_cv_name'); $results = tripal_core_chado_select('cv', array('*'), $select, $options); $syncv = $results[0]; } else { $syncv = $results[0]; } // get the 'exact' cvterm, which is the type of synonym we're adding $select = array( 'name' => 'exact', 'cv_id' => array( 'name' => 'synonym_type' ), ); $options = array('statement_name' => 'sel_cvterm_name_cvid'); $result = tripal_core_chado_select('cvterm', array('*'), $select, $options); $syntype = $result[0]; if (!$syntype) { $term = array( 'name' => 'exact', 'id' => "internal:exact", 'definition' => '', 'is_obsolete' => 0, ); // TODO: fix the function so it uses prepared statements $syntype = tripal_cv_add_cvterm($term, $syncv->name, 0, 1); if (!$syntype) { watchdog("T_gff3_loader", "Cannot add synonym type: internal:$type", array(), WATCHDOG_WARNING); return 0; } } // iterate through all of the aliases and add each one foreach ($aliases as $alias) { // check to see if the alias already exists in the synonym table // if not, then add it $select = array( 'name' => $alias, 'type_id' => $syntype->cvterm_id, ); $options = array('statement_name' => 'sel_synonym_name_typeid'); $result = tripal_core_chado_select('synonym', array('*'), $select, $options); $synonym = $result[0]; if (!$synonym) { $values = array( 'name' => $alias, 'type_id' => $syntype->cvterm_id, 'synonym_sgml' => '', ); $options = array('statement_name' => 'ins_synonym_name_typeid_synonymsgml'); $result = tripal_core_chado_insert('synonym', $values, $options); if (!$result) { watchdog("T_gff3_loader", "Cannot add alias $alias to synonym table", array(), WATCHDOG_WARNING); } } $options = array('statement_name' => 'sel_synonym_name_typeid'); $result = tripal_core_chado_select('synonym', array('*'), $select, $options); $synonym = $result[0]; // check to see if we have a NULL publication in the pub table. If not, // then add one. // @coder-ignore: non-drupal schema thus table prefixing does not apply $select = array('uniquename' => 'null'); $options = array('statement_name' => 'sel_pub_uniquename'); $result = tripal_core_chado_select('pub', array('*'), $select, $options); $pub = $result[0]; if (!$pub) { // prepare the statement if (!tripal_core_is_sql_prepared('ins_pub_uniquename_typeid')) { $psql = "PREPARE ins_pub_uniquename_typeid (text, text) AS INSERT INTO pub (uniquename,type_id) VALUES ('%s', (SELECT cvterm_id FROM cvterm CVT INNER JOIN dbxref DBX on DBX.dbxref_id = CVT.dbxref_id INNER JOIN db DB on DB.db_id = DBX.db_id WHERE CVT.name = $1 and DB.name = $2)"; $status = chado_query($psql); if (!$status) { watchdog("T_gff3_loader", "Cannot prepare statement 'ins_pub_uniquename_typeid", array(), WATCHDOG_WARNING); return 0; } } // insert the null pub $result = db_fetch_object(chado_query("EXECUTE ins_pub_uniquename_typeid ('%s', '%s')", 'null', 'null')); if (!$result) { watchdog("T_gff3_loader", "Cannot add null publication needed for setup of alias", array(), WATCHDOG_WARNING); return 0; } } $options = array('statement_name' => 'sel_pub_uniquename'); $result = tripal_core_chado_select('pub', array('*'), $select, $options); $pub = $result[0]; // check to see if the synonym exists in the feature_synonym table // if not, then add it. $select = array( 'synonym_id' => $synonym->synonym_id, 'feature_id' => $feature->feature_id, 'pub_id' => $pub->pub_id, ); $options = array('statement_name', 'sel_synonymfeature_synonymid_featureid_pubid'); $result = tripal_core_chado_select('feature_synonym', array('*'), $select, $options); $fsyn = $result[0]; if (!$fsyn) { $values = array( 'synonym_id' => $synonym->synonym_id, 'feature_id' => $feature->feature_id, 'pub_id' => $pub->pub_id, ); $options = array('statement_name', 'ins_synonymfeature_synonymid_featureid_pubid'); $result = tripal_core_chado_insert('feature_synonym', $values, $options); if (!$result) { watchdog("T_gff3_loader", "Cannot add alias $alias to feature synonym table", array(), WATCHDOG_WARNING); return 0; } } } return 1; } /** * * * @ingroup gff3_loader */ function tripal_feature_load_gff3_feature($organism, $analysis_id, $cvterm, $uniquename, $name, $residues, $is_analysis = 'f', $is_obsolete = 'f', $add_only, $score) { // check to see if the feature already exists $fselect = array( 'organism_id' => $organism->organism_id, 'uniquename' => $uniquename, 'type_id' => $cvterm->cvterm_id ); $options = array('statement_name' => 'sel_feature_orunty'); $result = tripal_core_chado_select('feature', array('*'), $fselect, $options); $feature = $result[0]; if (strcmp($is_obsolete, 'f')==0 or $is_obsolete == 0) { $is_obsolete = 'FALSE'; } if (strcmp($is_obsolete, 't')==0 or $is_obsolete == 1) { $is_obsolete = 'TRUE'; } if (strcmp($is_analysis, 'f')==0 or $is_analysis == 0) { $is_analysis = 'FALSE'; } if (strcmp($is_analysis, 't')==0 or $is_analysis == 1) { $is_analysis = 'TRUE'; } // insert the feature if it does not exist otherwise perform an update if (!$feature) { $values = array( 'organism_id' => $organism->organism_id, 'name' => $name, 'uniquename' => $uniquename, 'residues' => $residues, 'seqlen' => drupal_strlen($residues), 'md5checksum' => md5($residues), 'type_id' => $cvterm->cvterm_id, 'is_analysis' => $is_analysis, 'is_obsolete' => $is_obsolete, ); $options = array('statement_name' => 'ins_feature_all'); $result = tripal_core_chado_insert('feature', $values, $options); if (!$result) { watchdog("T_gff3_loader", "Failed to insert feature '$uniquename' ($cvterm->name)", array(), WATCHDOG_WARNING); return 0; } } elseif (!$add_only) { $values = array( 'name' => $name, 'residues' => $residues, 'seqlen' => drupal_strlen($residues), 'md5checksum' => md5($residues), 'is_analysis' => $is_analysis, 'is_obsolete' => $is_obsolete, ); $match = array( 'organism_id' => $organism->organism_id, 'uniquename' => $uniquename, 'type_id' => $cvterm->cvterm_id, ); $options = array('statement_name' => 'upd_feature'); $result = tripal_core_chado_update('feature', $match, $values, $options); if (!$result) { watchdog("T_gff3_loader", "Failed to update feature '$uniquename' ($cvterm->name)", array(), WATCHDOG_WARNING); return 0; } } else { // the feature exists and we don't want to update it so return // a value of 0. This will stop all downstream property additions return 0; } // get the newly added feature $options = array('statement_name' => 'sel_feature_orunty'); $result = tripal_core_chado_select('feature', array('*'), $fselect, $options); $feature = $result[0]; // add the analysisfeature entry to the analysisfeature table if it doesn't already exist $af_values = array( 'analysis_id' => $analysis_id, 'feature_id' => $feature->feature_id ); $options = array('statement_name' => 'sel_analysisfeature_analysisid_featureid'); $afeature = tripal_core_chado_select('analysisfeature', array('analysisfeature_id'), $af_values, $options); if (count($afeature)==0) { // if a score is available then set that to be the significance field if (strcmp($score, '.') != 0) { $af_values['significance'] = $score; $options = array('statement_name' => 'ins_analysisfeature_analysisid_featureid_significance'); } else { $options = array('statement_name' => 'ins_analysisfeature_analysisid_featureid'); } if (!tripal_core_chado_insert('analysisfeature', $af_values, $options)) { watchdog("T_gff3_loader", "Could not add analysisfeature record: $analysis_id, $feature->feature_id", array(), WATCHDOG_WARNING); } } else { // if a score is available then set that to be the significance field $new_vals = array(); if (strcmp($score, '.')!=0) { $new_vals['significance'] = $score; } else { $new_vals['significance'] = '__NULL__'; } if (!$add_only) { $options = array('statement_name' => 'upd_analysisfeature'); $ret = tripal_core_chado_update('analysisfeature', $af_values, $new_vals, $options); if (!$ret) { watchdog("T_gff3_loader", "Could not update analysisfeature record: $analysis_id, $feature->feature_id", array(), WATCHDOG_WARNING); } } } return $feature; } /** * * * @ingroup gff3_loader */ function tripal_feature_load_gff3_featureloc($feature, $organism, $landmark, $fmin, $fmax, $strand, $phase, $is_fmin_partial, $is_fmax_partial, $residue_info, $locgroup) { $select = array( 'organism_id' => $organism->organism_id, 'uniquename' => $landmark, ); $options = array('statement_name' => 'sel_feature_organism_id_uniquename'); $r = tripal_core_chado_select('feature', array('*'), $select, $options); if (count($r)==0) { // so we couldn't find it using the uniquename. Let's try the 'name'. // if we return only a singe result then we can proceed. Otherwise give an // error message $select = array( 'organism_id' => $organism->organism_id, 'name' => $landmark, ); $options = array('statement_name' => 'sel_feature_organism_id_name'); $r = tripal_core_chado_select('feature', array('*'), $select, $options); if (count($r) == 0){ watchdog("T_gff3_loader", "Cannot find landmark feature: '$landmark'. Cannot add the feature location record", array(), WATCHDOG_WARNING); return 0; } elseif (count($r) > 1) { watchdog("T_gff3_loader", "multiple landmarks exist with the name: '$landmark'. Cannot resolve which one to use. Cannot add the feature location record", array(), WATCHDOG_WARNING); return 0; } } $srcfeature = $r[0]; // TODO: create an attribute that recognizes the residue_info,locgroup, // is_fmin_partial and is_fmax_partial, right now these are // hardcoded to be false and 0 below. // check to see if this featureloc already exists, but also keep track of the // last rank value $rank = 0; $exists = 0; $select = array('feature_id' => $feature->feature_id); $options = array( 'statement_name' => 'sel_featureloc_feature_id', 'order_by' => array( 'rank' => 'ASC' ), ); $locrecs = tripal_core_chado_select('featureloc', array('*'), $select, $options); foreach ($locrecs as $featureloc) { $select = array('feature_id' => $featureloc->srcfeature_id); $options = array('statement_name' => 'sel_feature_feature_id'); $locsfeature = tripal_core_chado_select('feature', array('*'), $select, $options); // check to make sure we don't already have this featureloc record // if we do we don't want to readd it // print "Src Name:'" . $locsfeature[0]->name . "' == '$landmark'\n"; // print "Fmin: '$featureloc->fmin' == '$fmin'\n"; // print "Fmax: '$featureloc->fmax' == '$fmax'\n"; // print "Strand: '$featureloc->strand' == '$strand'\n"; // the source feature name and at least the fmin and fmax must be the same // for an update of the featureloc, otherwise we'll insert a new record. if (strcmp($locsfeature[0]->name, $landmark)==0 and ($featureloc->fmin == $fmin or $featureloc->fmax == $fmax)) { $match = array('featureloc_id' => $featureloc->featureloc_id); $values = array(); $exists = 1; if ($featureloc->fmin != $fmin) { $values['fmin'] = $fmin; } if ($featureloc->fmax != $fmax) { $values['fmax'] = $fmax; } if ($featureloc->strand != $strand) { $values['strand'] = $strand; } if (count($values) > 0) { $options = array('statement_name' => 'upd_featureloc_all'); tripal_core_chado_update('featureloc', $match, $values, $options); } } $rank = $featureloc->rank + 1; } if (!$exists) { // this feature location is new so add it if (strcmp($is_fmin_partial, 'f')==0 or !$is_fmin_partial) { $is_fmin_partial = 'FALSE'; } elseif (strcmp($is_fmin_partial, 't')==0 or $is_fmin_partial = 1) { $is_fmin_partial = 'TRUE'; } if (strcmp($is_fmax_partial, 'f')==0 or !$is_fmax_partial) { $is_fmax_partial = 'FALSE'; } elseif (strcmp($is_fmax_partial, 't')==0 or $is_fmax_partial = 1) { $is_fmax_partial = 'TRUE'; } $values = array( 'feature_id' => $feature->feature_id, 'srcfeature_id' => $srcfeature->feature_id, 'fmin' => $fmin, 'is_fmin_partial' => $is_fmin_partial, 'fmax' => $fmax, 'is_fmax_partial' => $is_fmax_partial, 'strand' => $strand, 'residue_info' => $residue_info, 'locgroup' => $locgroup, 'rank' => $rank ); $options = array('statement_name' => 'ins_featureloc_all'); if($phase) { $values['phase'] = $phase; $options = array('statement_name' => 'ins_featureloc_allphase'); } $success = tripal_core_chado_insert('featureloc', $values, $options); if (!$success) { watchdog("T_gff3_loader", "Failed to insert featureloc", array(), WATCHDOG_WARNING); exit; return 0; } } return 1; } /** * * * @ingroup gff3_loader */ function tripal_feature_load_gff3_property($feature, $property, $value) { // first make sure the cvterm exists. if not, then add it $select = array( 'name' => $property, 'cv_id' => array( 'name' => 'feature_property', ), ); $options = array('statement_name' => 'sel_cvterm_name_cvid'); $result = tripal_core_chado_select('cvterm', array('*'), $select, $options); $cvterm = $result[0]; if (!$cvterm) { $term = array( 'id' => "null:$property", 'name' => $property, 'namespace' => 'feature_property', 'is_obsolete' => 0, ); $cvterm = (object) tripal_cv_add_cvterm($term, 'feature_property', 0, 0); } if (!$cvterm) { watchdog("T_gff3_loader", "Cannot add cvterm, $property", array(), WATCHDOG_WARNING); exit; } // check to see if the property already exists for this feature // if it does but the value is unique then increment the rank and add it. // if the value is not unique then don't add it. $add = 1; $rank = 0; $select = array( 'feature_id' => $feature->feature_id, 'type_id' => $cvterm->cvterm_id, ); $options = array( 'statement_name' => 'sel_featureprop_featureid_typeid', 'order_by' => array( 'rank' => 'ASC', ), ); $results = tripal_core_chado_select('featureprop', array('*'), $select, $options); foreach ($results as $prop){ if (strcmp($prop->value, $value)==0) { $add = NULL; // don't add it, it already exists } $rank = $prop->rank + 1; } // add the property if we pass the check above if ($add) { $values = array( 'feature_id' => $feature->feature_id, 'type_id' => $cvterm->cvterm_id, 'value' => $value, 'rank' => $rank, ); $options = array('statement_name' => 'ins_featureprop_all'); $result = tripal_core_chado_insert('featureprop', $values, $options); if(!$result){ watchdog("T_gff3_loader", "cannot add featureprop, $property", array(), WATCHDOG_WARNING); } } }