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@@ -1,96 +1,148 @@
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<?php
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-function blast_ui_services_resources() {
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- return array(
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- 'blast' => array(
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- 'retrieve' => array(
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- 'help' => 'Retrieves a blast_ui',
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- 'callback' => '_blast_ui_retrieve',
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- 'access callback' => 'user_access',
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- 'access arguments' => array('access user profiles'),
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- 'args' => array(),
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- ),
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- 'create' => array(
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- 'help' => 'Creates a blast_ui',
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- 'callback' => '_blast_ui_create',
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- 'access arguments' => array('access content'),
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- 'access arguments append' => false,
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- 'args' => array(
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- array(
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- 'name' => 'param',
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- 'type' => 'array',
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- 'description' => 'The node information',
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- 'source' => 'data',
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- 'optional' => FALSE,
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- ),
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- ),
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- ),
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- 'update' => array(
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- 'help' => 'Updates a blast_ui',
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- 'callback' => '_blast_ui_update',
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- 'access callback' => 'user_access',
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- 'access arguments' => array('access user profiles'),
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- 'args' => array(
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- array(
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- 'name' => 'id',
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- 'type' => 'int',
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- 'description' => 'The id of the node to update',
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- 'source' => array('path' => '0'),
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- 'optional' => FALSE,
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- ),
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- array(
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- 'name' => 'data',
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- 'type' => 'struct',
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- 'description' => 'The node data object',
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- 'source' => 'data',
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- 'optional' => FALSE,
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- ),
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- ),
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- ),
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- 'delete' => array(
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- 'help' => 'Deletes a blast_ui',
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- 'callback' => '_blast_ui_delete',
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- 'access callback' => 'user_access',
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- 'access arguments' => array('access content'),
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- 'access arguments append' => TRUE,
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- 'args' => array(
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- array(
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- 'name' => 'nid',
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- 'type' => 'int',
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- 'description' => 'The id of the note to delete',
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- 'source' => array('path' => '0'),
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- 'optional' => FALSE,
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- ),
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- ),
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- ),
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- 'index' => array(
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- 'help' => 'Retrieves a listing of blast_ui',
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- 'callback' => '_blast_ui_index',
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- 'access callback' => 'user_access',
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- 'access arguments' => array('access content'),
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- 'access arguments append' => FALSE,
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- 'args' => array(),
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- ),
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-
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- 'actions' => array(
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- 'getDatabaseType' => array(
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- 'help' => 'Retrieves a listing of database',
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- 'callback' => '_blast_ui_getDatabaseType',
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- 'access callback' => 'user_access',
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- 'access arguments' => array('access content'),
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- 'access arguments append' => FALSE,
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- 'args' => array(),
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- ),
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- 'getDatabaseOptions' => array(
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- 'help' => 'Retrieves a listing of database',
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- 'callback' => '_blast_ui_getDatabaseOption',
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- 'access callback' => 'user_access',
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- 'access arguments' => array('access content'),
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- 'access arguments append' => FALSE,
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- 'args' => array(),
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- ),
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- ),
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- ),
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- );
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+/**
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+* Implements hook_services_resources().
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+* Hooks provided by Services for the definition of new services,
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+*/
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+
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+function blast_ui_services_resources()
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+{
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+ return array(
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+ 'blast' => array(
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+ 'retrieve' => array(
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+ 'help' => 'Retrieves a blastResult',
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+ 'callback' => 'getBlastData',
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+ 'access callback' => 'user_access',
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+ 'access arguments' => array(
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+ 'access content'
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+ ),
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+ 'access arguments append' => FALSE,
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+ 'args' => array(
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+ array(
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+ 'name' => 'job_id',
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+ 'type' => 'int',
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+ 'description' => 'The information needed',
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+ 'source' => array(
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+ 'path' => '0'
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+ ),
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+ 'optional' => FALSE
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+ )
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+ )
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+ ),
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+ 'create' => array(
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+ 'help' => 'Creates a blast_ui',
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+ 'callback' => '_blast_ui_create',
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+ 'access arguments' => array(
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+ 'access content'
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+ ),
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+ 'access arguments append' => false,
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+ 'args' => array(
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+ array(
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+ 'name' => 'param',
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+ 'type' => 'array',
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+ 'description' => 'The information needed',
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+ 'source' => 'data',
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+ 'optional' => FALSE
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+ )
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+ )
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+ ),
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+ 'update' => array(
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+ 'help' => 'Updates a blast_ui',
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+ 'callback' => '_blast_ui_update',
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+ 'access callback' => 'user_access',
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+ 'access arguments' => array(
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+ 'access user profiles'
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+ ),
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+ 'args' => array(
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+ array(
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+ 'name' => 'id',
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+ 'type' => 'int',
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+ 'description' => 'The information needed',
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+ 'source' => array(
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+ 'path' => '0'
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+ ),
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+ 'optional' => FALSE
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+ ),
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+ array(
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+ 'name' => 'data',
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+ 'type' => 'struct',
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+ 'description' => 'The information needed',
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+ 'source' => 'data',
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+ 'optional' => FALSE
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+ )
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+ )
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+ ),
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+ 'delete' => array(
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+ 'help' => 'Deletes a blast_ui',
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+ 'callback' => '_blast_ui_delete',
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+ 'access callback' => 'user_access',
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+ 'access arguments' => array(
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+ 'access content'
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+ ),
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+ 'access arguments append' => TRUE,
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+ 'args' => array(
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+ array(
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+ 'name' => 'nid',
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+ 'type' => 'int',
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+ 'description' => 'The information needed',
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+ 'source' => array(
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+ 'path' => '0'
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+ ),
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+ 'optional' => FALSE
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+ )
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+ )
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+ ),
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+ 'index' => array(
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+ 'help' => 'Retrieves a listing of blast_ui',
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+ 'callback' => '_blast_ui_index',
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+ 'access callback' => 'user_access',
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+ 'access arguments' => array(
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+ 'access content'
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+ ),
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+ 'access arguments append' => FALSE,
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+ 'args' => array()
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+ ),
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+
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+ 'actions' => array(
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+ 'getJobId' => array(
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+ 'help' => 'Retrieves a listing of database',
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+ 'callback' => '_blast_ui_getJobId',
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+ 'access callback' => 'user_access',
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+ 'access arguments' => array(
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+ 'access content'
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+ ),
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+ 'args' => array(
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+ array(
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+ 'name' => 'data',
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+ 'type' => 'array',
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+ 'description' => 'Retrieve blast job id',
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+ 'source' => 'data',
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+ 'optional' => FALSE
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+ )
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+ ),
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+ 'access arguments append' => FALSE
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+ ),
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+
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+ 'getDatabaseOptions' => array(
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+ 'help' => 'Retrieves a listing of database',
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+ 'callback' => '_blast_ui_getDatabaseOption',
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+ 'access callback' => 'user_access',
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+ 'access arguments' => array(
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+ 'access content'
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+ ),
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+ 'args' => array(
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+ array(
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+ 'name' => 'data',
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+ 'type' => 'array',
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+ 'description' => 'The set database_type information',
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+ 'source' => 'data',
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+ 'optional' => FALSE
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+ )
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+ ),
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+ 'access arguments append' => false
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+ )
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+ )
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+ )
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+ );
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}
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@@ -98,77 +150,304 @@ function blast_ui_services_resources() {
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-function _blast_ui_create($param) {
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-
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- return services_error('Missing _blast_ui_create', 406);
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+function _blast_ui_create($param)
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+{
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+
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+ return services_error('Missing _blast_ui_create', 406);
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}
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/**
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-* Callback for updating note services.
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-*
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-* @param int $id
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-* @param object $data
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-* @return object
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-*/
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-function _blast_ui_update($id, $data) {
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- return services_error('Missing _blast_ui_update', 406);
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-}
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+ * Callback for updating blast services.
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+ *
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+ * @param int $id
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+ * @param object $data
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+ * @return object
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+ */
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+function _blast_ui_update($id, $data)
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+{
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+ return services_error('Missing _blast_ui_update', 406);
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+}
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/**
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-* Callback for retrieving note services.
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-*
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-* @param int $id
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-* @return object
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-*/
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-function _blast_ui_retrieve($id) {
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- return services_error('Missing _blast_ui_retrieve', 406);
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+ * Callback for retrieving blast services.
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+ *
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+ * @param int $id
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+ * @return object
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+ */
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+function _blast_ui_retrieve($id)
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+{
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+ return getBlastData($id);
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}
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/**
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-* Callback for deleting note services.
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-*
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-* @param int $id
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-* @return object
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-*/
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-function _blast_ui_delete($id) {
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- return services_error('Missing _blast_ui_delete', 406);
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+ * Callback for deleting blast services.
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+ *
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+ * @param int $id
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+ * @return object
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+ */
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+function _blast_ui_delete($id)
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+{
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+ return services_error('Missing _blast_ui_delete', 406);
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}
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-function _blast_ui_index($page, $parameters) {
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- return array(
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- 'Nucleotide' => array (
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- 'Nucleotide' => 'blastn',
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- 'protein' => 'blastx',
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- ),
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- 'Protein' => array (
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- 'Nucleotide' => 'tblastn',
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- 'protein' => 'blastp',
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- ),
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- );
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-
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-// return services_error('Missing _blast_ui_index solved', 406);
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+/**
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+ * Callback for Index blast services.
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+ */
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+
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+function _blast_ui_index()
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+{
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+ return array(
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+
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+ 'query_type' => array(
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+ '1' => 'nucleotide',
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+ '2' => 'protein'
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+ ),
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+ 'db_type' => array(
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+ '1' => 'nucleotide',
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+ '2' => 'protein'
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+ ),
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+ 'blast_program' => array(
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+ '1' => 'blastn',
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+ '2' => 'blastx',
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+ '3' => 'tblastn',
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+ '4' => 'blastp'
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+ )
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+
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+ );
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}
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-function _blast_ui_getDatabaseType() {
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- return array(
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- 'Nucleotide' => array (
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- 'Nucleotide' => 'blastn',
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- 'protein' => 'blastx',
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- ),
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- 'Protein' => array (
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- 'Nucleotide' => 'tblastn',
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- 'protein' => 'blastp',
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- ),
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- );
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+
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+function _blast_ui_getDatabaseOption($data)
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+{
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+
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+ if (!isset($data[ query_type ]))
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+ {
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+ return services_error('Missing blast attribute $query_type [ set necleotide or protein in post request of query_type ]', 406);
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+ }
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+ if (!isset($data[ db_type ]))
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+ {
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+ return services_error('Missing blast attribute $db_type [ set necleotide or protein in post request of db_type ]', 406);
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+ }
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+ if (!isset($data[ blast_program ]))
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+ {
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+ return services_error('Missing blast attribute $blast_program [ set blastn, blastx, tblastn, blastp in post request of blast_program ]', 406);
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+ }
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+
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+ $query_type = $data[ db_type ];
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+ $db_type = $data[ db_type ];
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+ $blast_program = $data[ blast_program ];
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+ $options_database = get_blast_database_options($db_type);
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+
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+
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+
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+ if ($blast_program == 'blastn')
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+ {
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+ return array(
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+ 'Select_Database' => $options_database,
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+ 'Max_target_sequences' => _get_max_target($blast_program),
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+ 'Word_size' => _get_word_size($blast_program),
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+ 'Match_Mismatch_Scores' => _get_match_mismatch($blast_program),
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+ 'Gap_Costs' => _get_gap($blast_program),
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+ 'Default_Values' => _get_default_values('')
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+ );
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+ }
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+ elseif ($blast_program == 'blastx')
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+ {
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+ return array(
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+ 'Select_Database' => $options_database,
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+ 'Max_target_sequences' => _get_max_target($blast_program),
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+ 'Word_size' => _get_word_size($blast_program),
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+ 'Matrix_options' => _get_matrix_options()
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+
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+ );
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+ }
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+}
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+
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+
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+function blast_validation($query_type, $db_type, $data)
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+{
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+
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+ if ($query_type == 'nucleotide')
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+ {
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+ if ($db_type == 'nucleotide')
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+ {
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+
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+ if (!isset($data[ Select_Database ]))
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+ {
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+ return services_error('Missing blast attribute $Select_Database [ query blast/getDatabaseOptions for options :- Add database you need to use]', 406);
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+ }
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+ if (!isset($data[ Max_target_sequences ]))
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+ {
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+ return services_error('Missing blast attribute $Max_target_sequences [ query blast/getDatabaseOptions for options ]', 406);
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+ }
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+ if (!isset($data[ Word_size ]))
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+ {
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+ return services_error('Missing blast attribute $Word_size [ query blast/getDatabaseOptions for options ]', 406);
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+ }
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+ if (!isset($data[ Match_Mismatch_Scores ]))
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+ {
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+ return services_error('Missing blast attribute $Match_Mismatch_Scores [ query blast/getDatabaseOptions for options]', 406);
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+ }
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+ if (!isset($data[ Gap_Costs ]))
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+ {
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+ return services_error('Missing blast attribute $Gap_Costs [ query blast/getDatabaseOptions for options ]', 406);
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+ }
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+ if (!isset($data[ Default_Values ]))
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+ {
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+ return services_error('Missing blast attribute $Default_Values [ query blast/getDatabaseOptions for options]', 406);
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+ }
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+ if (!isset($data[ seqQuery ]))
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+ {
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+ return services_error('Missing blast attribute $seqQuery [ Add sequence query for blast]', 406);
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+ }
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+
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+ }
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+ elseif ($db_type == 'protein')
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+ {
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+
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|
+ if (!isset($data[ Select_Database ]))
|
|
|
+ {
|
|
|
+ return services_error('Missing blast attribute $Select_Database [ query blast/getDatabaseOptions for options :- Add database used by you]', 406);
|
|
|
+ }
|
|
|
+ if (!isset($data[ Max_target_sequences ]))
|
|
|
+ {
|
|
|
+ return services_error('Missing blast attribute $Max_target_sequences [ query blast/getDatabaseOptions for options ]', 406);
|
|
|
+ }
|
|
|
+ if (!isset($data[ Word_size ]))
|
|
|
+ {
|
|
|
+ return services_error('Missing blast attribute $Word_size [ query blast/getDatabaseOptions for options ]', 406);
|
|
|
+ }
|
|
|
+ if (!isset($data[ Matrix_options ]))
|
|
|
+ {
|
|
|
+ return services_error('Missing blast attribute $Matrix_options [ query blast/getDatabaseOptions for options ]', 406);
|
|
|
+ }
|
|
|
+ if (!isset($data[ seqQuery ]))
|
|
|
+ {
|
|
|
+ return services_error('Missing blast attribute $seqQuery [ Add sequence query for blast]', 406);
|
|
|
+ }
|
|
|
+
|
|
|
+ }
|
|
|
+ }
|
|
|
+ /*
|
|
|
+ elseif ($query_type == 'protein') {
|
|
|
+ if ($db_type == 'nucleotide') {
|
|
|
+ $blast_program = 'tblastn';
|
|
|
+ }
|
|
|
+ elseif ($db_type == 'protein') {
|
|
|
+ $blast_program = 'blastp';
|
|
|
+ }
|
|
|
+ }
|
|
|
+
|
|
|
+
|
|
|
+ */
|
|
|
+
|
|
|
+}
|
|
|
+
|
|
|
+
|
|
|
+
|
|
|
+
|
|
|
+function _blast_ui_getJobId($data)
|
|
|
+{
|
|
|
+
|
|
|
+ $query_type;
|
|
|
+ $db_type;
|
|
|
+ $eVal = 0.001;
|
|
|
+ $qRange = 0;
|
|
|
+
|
|
|
+
|
|
|
+
|
|
|
+
|
|
|
+ /* check values in post query */
|
|
|
+
|
|
|
+ if (!isset($data[ query_type ]))
|
|
|
+ {
|
|
|
+ return services_error('Missing blast attribute $query_type [ set necleotide or protein in post request of query_type ]', 406);
|
|
|
+ }
|
|
|
+ if (!isset($data[ db_type ]))
|
|
|
+ {
|
|
|
+ return services_error('Missing blast attribute $db_type [ set necleotide or protein in post request of db_type ]', 406);
|
|
|
+ }
|
|
|
+
|
|
|
+ $query_type = $data[ query_type ];
|
|
|
+ $db_type = $data[ db_type ];
|
|
|
+
|
|
|
+ blast_validation($query_type, $db_type, $data);
|
|
|
+
|
|
|
+ if (isset($data[ $eVal ]))
|
|
|
+ {
|
|
|
+ $eVal = $data[ $eVal ];
|
|
|
+ }
|
|
|
+ if (isset($data[ $culling_limit ]))
|
|
|
+ {
|
|
|
+ $qRange = $data[ $culling_limit ];
|
|
|
+ }
|
|
|
+
|
|
|
+ if ($query_type == 'nucleotide')
|
|
|
+ {
|
|
|
+ if ($db_type == 'nucleotide')
|
|
|
+ {
|
|
|
+ $blast_program = 'blastn';
|
|
|
+ // code not working for wordvalue = 7 ?
|
|
|
+ $wordvalue = 11;
|
|
|
+ // $wordvalue = intval($data[Word_size]);
|
|
|
+ $numAlign = intval($data[ Max_target_sequences ]);
|
|
|
+ $options_database = get_blast_database_options($data[ db_type ]);
|
|
|
+ $Databasekey = array_search($data[ Select_Database ], $options_database);
|
|
|
+ $advanced_options = advanced_options($blast_program, $data[ Gap_Costs ], $data[ Match_Mismatch_Scores ], $wordvalue, $numAlign, $eVal, $qRange);
|
|
|
+
|
|
|
+ }
|
|
|
+ elseif ($db_type == 'protein')
|
|
|
+ {
|
|
|
+ $blast_program = 'blastx';
|
|
|
+ return 'working';
|
|
|
+ }
|
|
|
+ }
|
|
|
+ elseif ($query_type == 'protein')
|
|
|
+ {
|
|
|
+ if ($db_type == 'nucleotide')
|
|
|
+ {
|
|
|
+ $blast_program = 'tblastn';
|
|
|
+ }
|
|
|
+ elseif ($db_type == 'protein')
|
|
|
+ {
|
|
|
+ $blast_program = 'blastp';
|
|
|
+ }
|
|
|
+ }
|
|
|
+
|
|
|
+
|
|
|
+ return blast_ui_getblastJobId($data[ query_type ], $data[ db_type ], $data[ seqQuery ], $Databasekey, $advanced_options);
|
|
|
+
|
|
|
+
|
|
|
}
|
|
|
|
|
|
+// Define the advanced Options for Blast
|
|
|
|
|
|
-function _blast_ui_getDatabaseOption() {
|
|
|
|
|
|
- $db_type = 'nucleotide';
|
|
|
- $options = get_blast_database_options($db_type);
|
|
|
-return $options;
|
|
|
+function advanced_options($blast_program, $gapCost, $Match_Mismatch_Scores, $wordvalue, $numAlign, $eVal, $qRange)
|
|
|
+{
|
|
|
+
|
|
|
+ $Data_GapCost = _get_gap($blast_program);
|
|
|
+ $gapkey = array_search($gapCost, $Data_GapCost);
|
|
|
+ $gap = _set_gap($gapkey);
|
|
|
+
|
|
|
+
|
|
|
+ $Data_Mismatch_Scores = _get_match_mismatch($blast_program);
|
|
|
+ $Mismatch_Scores_key = array_search($Match_Mismatch_Scores, $Data_Mismatch_Scores);
|
|
|
+ $m_m = _set_match_mismatch($Mismatch_Scores_key);
|
|
|
+
|
|
|
+
|
|
|
+ return array(
|
|
|
+ 'max_target_seqs' => $numAlign,
|
|
|
+ 'evalue' => $eVal,
|
|
|
+ 'word_size' => $wordvalue,
|
|
|
+ 'gapopen' => $gap[ 'gapOpen' ],
|
|
|
+ 'gapextend' => $gap[ 'gapExtend' ],
|
|
|
+ 'penalty' => $m_m[ 'penalty' ],
|
|
|
+ 'reward' => $m_m[ 'reward' ],
|
|
|
+ 'culling_limit' => $qRange
|
|
|
+
|
|
|
+ );
|
|
|
}
|
|
|
|
|
|
|
|
@@ -177,6 +456,66 @@ return $options;
|
|
|
|
|
|
|
|
|
|
|
|
+function blast_ui_getblastJobId($query_type, $db_type, $seqQuery, $Databasekey, $adv_options)
|
|
|
+{
|
|
|
+
|
|
|
+
|
|
|
+
|
|
|
+ if ($query_type == 'nucleotide')
|
|
|
+ {
|
|
|
+ if ($db_type == 'nucleotide')
|
|
|
+ {
|
|
|
+ $blast_program = 'blastn';
|
|
|
+ }
|
|
|
+ elseif ($db_type == 'protein')
|
|
|
+ {
|
|
|
+ $blast_program = 'blastx';
|
|
|
+ }
|
|
|
+ }
|
|
|
+ elseif ($query_type == 'protein')
|
|
|
+ {
|
|
|
+ if ($db_type == 'nucleotide')
|
|
|
+ {
|
|
|
+ $blast_program = 'tblastn';
|
|
|
+ }
|
|
|
+ elseif ($db_type == 'protein')
|
|
|
+ {
|
|
|
+ $blast_program = 'blastp';
|
|
|
+ }
|
|
|
+ }
|
|
|
+
|
|
|
+ $query = '/tmp/' . date('YMd_His') . '_query.fasta';
|
|
|
+ file_put_contents($query, $seqQuery);
|
|
|
+ $blastdb_node = node_load($Databasekey);
|
|
|
+ $blastdb_name = $blastdb_node->db_name;
|
|
|
+ $blastdb_with_path = $blastdb_node->db_path;
|
|
|
+
|
|
|
+ // Now let each program process its own advanced options.
|
|
|
+
|
|
|
+
|
|
|
+ $advanced_options = $adv_options;
|
|
|
+ global $user;
|
|
|
+
|
|
|
+ $output_filestub = date('YMd_His');
|
|
|
+ $job_args = array(
|
|
|
+ 'program' => $blast_program,
|
|
|
+ 'query' => $query,
|
|
|
+ 'database' => $blastdb_with_path,
|
|
|
+ 'output_filename' => $output_filestub,
|
|
|
+ 'options' => $advanced_options
|
|
|
+ );
|
|
|
+
|
|
|
+ $job_id = tripal_add_job(t('BLAST (@program): @query', array(
|
|
|
+ '@program' => $blast_program,
|
|
|
+ '@query' => $query
|
|
|
+ )), 'blast_job', 'run_BLAST_tripal_job', $job_args, $user->uid);
|
|
|
+
|
|
|
+ ob_start();
|
|
|
+ tripal_launch_job(1, $job_id);
|
|
|
+ ob_end_clean();
|
|
|
+ return $job_id;
|
|
|
+ // return getBlastData($job_id);
|
|
|
+}
|
|
|
|
|
|
|
|
|
|
|
@@ -188,5 +527,37 @@ return $options;
|
|
|
|
|
|
|
|
|
|
|
|
+function getBlaststatus($job_id)
|
|
|
+{
|
|
|
+ /* $sql = "SELECT status FROM tripal_jobs WHERE job_id = :job_id ";
|
|
|
+ $job_res = db_query($sql, array(':job_id' => $job_id))->fetchField();
|
|
|
+ return $job_res; */
|
|
|
+}
|
|
|
+
|
|
|
+
|
|
|
+
|
|
|
+function getBlastData($job_id)
|
|
|
+{
|
|
|
+ $result;
|
|
|
+ $sql = "SELECT job_name FROM tripal_jobs WHERE job_id = :job_id ";
|
|
|
+ $job_res = db_query($sql, array(
|
|
|
+ ':job_id' => $job_id
|
|
|
+ ))->fetchField();
|
|
|
+ $file_path = str_replace('_query.fasta', '.blast.asn', $job_res);
|
|
|
+ $file_path = str_replace('BLAST (blastn): /', '/', $file_path);
|
|
|
+
|
|
|
+ if (file_exists($file_path))
|
|
|
+ {
|
|
|
+ $result = file_get_contents($file_path);
|
|
|
+ }
|
|
|
+ else
|
|
|
+ {
|
|
|
+ $result = "The file $file_path does not exist";
|
|
|
+ }
|
|
|
+ return $result;
|
|
|
+}
|
|
|
+
|
|
|
+
|
|
|
+
|
|
|
|
|
|
|