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@@ -1,658 +0,0 @@
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-<?php
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-/**
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- * Implements hook_services_resources().
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- * Hooks provided by Services for the definition of new services,
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- */
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-
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-function blast_ui_services_resources()
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- {
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- return array(
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- 'blast' => array(
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- 'retrieve' => array(
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- 'help' => 'Retrieves a blastResult',
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- 'callback' => 'getBlastData',
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- 'access callback' => 'user_access',
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- 'access arguments' => array(
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- 'access content'
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- ),
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- 'access arguments append' => FALSE,
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- 'args' => array(
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- array(
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- 'name' => 'job_id',
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- 'type' => 'int',
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- 'description' => 'The information needed',
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- 'source' => array(
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- 'path' => '0'
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- ),
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- 'optional' => FALSE
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- )
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- )
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- ),
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- 'index' => array(
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- 'help' => 'Retrieves a listing of blast_ui',
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- 'callback' => '_blast_ui_index',
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- 'access callback' => 'user_access',
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- 'access arguments' => array(
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- 'access content'
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- ),
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- 'access arguments append' => FALSE,
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- 'args' => array ()
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- ),
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-
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- 'actions' => array(
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- 'getJobId' => array(
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- 'help' => 'Retrieves a listing of database',
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- 'callback' => '_blast_ui_getJobId',
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- 'access callback' => 'user_access',
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- 'access arguments' => array(
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- 'access content'
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- ),
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- 'args' => array(
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- array(
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- 'name' => 'data',
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- 'type' => 'array',
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- 'description' => 'Retrieve blast job id',
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- 'source' => 'data',
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- 'optional' => FALSE
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- )
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- ),
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- 'access arguments append' => FALSE
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- ),
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-
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- 'getDatabaseOptions' => array(
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- 'help' => 'Retrieves a listing of database',
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- 'callback' => '_blast_ui_getDatabaseOption',
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- 'access callback' => 'user_access',
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- 'access arguments' => array(
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- 'access content'
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- ),
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- 'args' => array(
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- array(
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- 'name' => 'data',
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- 'type' => 'array',
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- 'description' => 'The set database_type information',
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- 'source' => 'data',
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- 'optional' => FALSE
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- )
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- ),
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- 'access arguments append' => false
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- )
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- )
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- )
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- );
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- }
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-
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-
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-
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-/**
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- * Callback for retrieving blast services.
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- *
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- * @param int $id
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- * @return object
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- */
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-function _blast_ui_retrieve( $id )
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- {
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- return getBlastData( $id );
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- }
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-
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-/**
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- * Callback for Index blast services.
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- * @return object
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- */
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-
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-function _blast_ui_index()
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- {
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- return array(
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-
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- 'query_type' => array(
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- '1' => 'nucleotide',
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- '2' => 'protein'
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- ),
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- 'db_type' => array(
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- '1' => 'nucleotide',
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- '2' => 'protein'
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- ),
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- 'blast_program' => array(
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- '1' => 'blastn',
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- '2' => 'blastx',
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- '3' => 'tblastn',
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- '4' => 'blastp'
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- )
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- );
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- }
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-
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-/*
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- ** Method returns Advanced options and Database Type
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- ** @param Object $data
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- ** web service url : http://localhost:8080/restapi/blast/getDatabaseOptions.json
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- */
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-
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-function _blast_ui_getDatabaseOption( $data )
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- {
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-
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- if ( !isset( $data[ query_type ] ) )
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- {
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- return services_error( 'Missing blast attribute $query_type [ set necleotide or protein in post request of query_type ]', 406 );
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- }
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- if ( !isset( $data[ db_type ] ) )
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- {
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- return services_error( 'Missing blast attribute $db_type [ set necleotide or protein in post request of db_type ]', 406 );
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- }
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- if ( !isset( $data[ blast_program ] ) )
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- {
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- return services_error( 'Missing blast attribute $blast_program [ set blastn, blastx, tblastn, blastp in post request of blast_program ]', 406 );
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- }
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-
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- $query_type = $data[ db_type ];
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- $db_type = $data[ db_type ];
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- $blast_program = $data[ blast_program ];
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- $options_database = get_blast_database_options( $db_type );
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-
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- // Data Set send to user for selection of Blastn
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- if ( $blast_program == 'blastn' )
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- {
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- return array(
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- 'Select_Database' => $options_database,
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- 'Max_target_sequences' => _get_max_target( $blast_program ),
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- 'Word_size' => _get_word_size( $blast_program ),
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- 'Match_Mismatch_Scores' => _get_match_mismatch( $blast_program ),
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- 'Gap_Costs' => _get_gap( $blast_program )
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- );
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- }
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- // Data Set send to user for selection of Blastx
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- elseif ( $blast_program == 'blastx' )
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- {
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- return array(
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- 'Select_Database' => $options_database,
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- 'Max_target_sequences' => _get_max_target( $blast_program ),
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- 'Word_size' => _get_word_size( $blast_program ),
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- 'Matrix_options' => _get_matrix_options()
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-
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- );
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- }
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- //Data Set send to user for selection of tblastn
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- elseif ( $blast_program == 'tblastn' )
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- {
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- return array(
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- 'Select_Database' => $options_database,
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- 'Max_target_sequences' => _get_max_target( $blast_program ),
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- 'Word_size' => _get_word_size( $blast_program ),
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- 'Matrix_options' => _get_matrix_options(),
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- 'Gap_Costs' => getGap_cost()
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- );
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- }
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- //Data Set send to user for selection of blastp
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- elseif ( $blast_program == 'blastp' )
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- {
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- return array(
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- 'Select_Database' => $options_database,
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- 'Max_target_sequences' => _get_max_target( $blast_program ),
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- 'Word_size' => _get_word_size( $blast_program ),
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- 'Matrix_options' => _get_matrix_options(),
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- 'Gap_Costs' => getGap_cost()
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- );
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- }
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- }
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-
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-/*
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- ** validate the data
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- */
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-
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-function blast_validation( $query_type, $db_type, $data )
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- {
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- if ( $query_type == 'nucleotide' )
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- {
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- if ( $db_type == 'nucleotide' )
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- {
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-
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- if ( !isset( $data[ Select_Database ] ) )
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- {
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- return services_error( 'Missing blast attribute $Select_Database [ query blast/getDatabaseOptions for options :- Add database you need to use]', 406 );
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- }
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- if ( !isset( $data[ Max_target_sequences ] ) )
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- {
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- return services_error( 'Missing blast attribute $Max_target_sequences [ query blast/getDatabaseOptions for options ]', 406 );
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- }
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- if ( !isset( $data[ Word_size ] ) )
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- {
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- return services_error( 'Missing blast attribute $Word_size [ query blast/getDatabaseOptions for options ]', 406 );
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- }
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- if ( !isset( $data[ Match_Mismatch_Scores ] ) )
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- {
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- return services_error( 'Missing blast attribute $Match_Mismatch_Scores [ query blast/getDatabaseOptions for options]', 406 );
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- }
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- if ( !isset( $data[ Gap_costs ] ) )
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- {
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- return services_error( 'Missing blast attribute $Gap_Costs [ query blast/getDatabaseOptions for options ]', 406 );
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- }
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- if ( isset( $data[ seqQuery ] ) )
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- {
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- if ( validate_fasta_sequence( $query_type, $data[ seqQuery ] ) == 1 )
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- {
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- return services_error( 'Please enter validate_fasta_sequence', 406 );
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- }
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- }
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- else
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- {
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- return services_error( 'Missing blast attribute data[seqQuery]', 406 );
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- }
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- }
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- else
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- {
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-
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- if ( !isset( $data[ Select_Database ] ) )
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- {
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- return services_error( 'Missing blast attribute $Select_Database [ query blast/getDatabaseOptions for options :- Add database used by you]', 406 );
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- }
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- if ( !isset( $data[ Max_target_sequences ] ) )
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- {
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- return services_error( 'Missing blast attribute $Max_target_sequences [ query blast/getDatabaseOptions for options ]', 406 );
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- }
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- if ( !isset( $data[ Word_size ] ) )
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- {
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- return services_error( 'Missing blast attribute $Word_size [ query blast/getDatabaseOptions for options ]', 406 );
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- }
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- if ( !isset( $data[ Matrix_options ] ) )
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- {
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- return services_error( 'Missing blast attribute $Matrix_options [ query blast/getDatabaseOptions for options ]', 406 );
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- }
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- if ( isset( $data[ seqQuery ] ) )
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- {
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- if ( validate_fasta_sequence( $query_type, $data[ seqQuery ] ) == 1 )
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- {
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- return services_error( 'Please enter validate_fasta_sequence', 406 );
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- }
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- }
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- else
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- {
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- return services_error( 'Missing blast attribute data[seqQuery]', 406 );
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- }
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-
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- }
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- }
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- else
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- {
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- if ( $db_type == 'nucleotide' )
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- {
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- if ( !isset( $data[ Select_Database ] ) )
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- {
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- return services_error( 'Missing blast attribute $Select_Database [ query blast/getDatabaseOptions for options :- Add database you need to use]', 406 );
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- }
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- if ( !isset( $data[ Max_target_sequences ] ) )
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- {
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- return services_error( 'Missing blast attribute $Max_target_sequences [ query blast/getDatabaseOptions for options ]', 406 );
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- }
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- if ( !isset( $data[ Word_size ] ) )
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- {
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- return services_error( 'Missing blast attribute $Word_size [ query blast/getDatabaseOptions for options ]', 406 );
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- }
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- if ( !isset( $data[ Matrix_options ] ) )
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- {
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- return services_error( 'Missing blast attribute $Matrix_options [ query blast/getDatabaseOptions for options ]', 406 );
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- }
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- if ( !isset( $data[ Gap_costs ] ) )
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- {
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- return services_error( 'Missing blast attribute [ query blast/getDatabaseOptions for options ]', 406 );
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- }
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-
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- if ( isset( $data[ seqQuery ] ) )
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- {
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- if ( validate_fasta_sequence( $query_type, $data[ seqQuery ] ) == 1 )
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- {
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- return services_error( 'Please enter validate_fasta_sequence', 406 );
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- }
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- }
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- else
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- {
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- return services_error( 'Missing blast attribute data[seqQuery]', 406 );
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- }
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- }
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- else
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- {
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-
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- //return $data;
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-
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- if ( !isset( $data[ Select_Database ] ) )
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- {
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- return services_error( 'Missing blast attribute $Select_Database [ query blast/getDatabaseOptions for options :- Add database used by you]', 406 );
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- }
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- if ( !isset( $data[ Max_target_sequences ] ) )
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- {
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- return services_error( 'Missing blast attribute $Max_target_sequences [ query blast/getDatabaseOptions for options ]', 406 );
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- }
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- if ( !isset( $data[ Word_size ] ) )
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- {
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- return services_error( 'Missing blast attribute $Word_size [ query blast/getDatabaseOptions for options ]', 406 );
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- }
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- if ( !isset( $data[ Matrix_options ] ) )
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- {
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- return services_error( 'Missing blast attribute $Matrix_options [ query blast/getDatabaseOptions for options ]', 406 );
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- }
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- if ( isset( $data[ seqQuery ] ) )
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- {
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- if ( validate_fasta_sequence( $query_type, $data[ seqQuery ] ) == 1 )
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- {
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- return services_error( 'Please enter validate_fasta_sequence', 406 );
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- }
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- }
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- else
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- {
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- return services_error( 'Missing blast attribute data[seqQuery]', 406 );
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- }
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- }
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- }
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- }
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-
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-/*
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- ** Method call the blast_ui_getblastJobId by setting all the needed
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- ** parameters
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- ** web service url : http://localhost:8080/restapi/blast/getJobId.json
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- */
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-
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-
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-function _blast_ui_getJobId( $data )
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- {
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-
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- $query_type;
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- $db_type;
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- // Setting the default value of qRange and eValue from blast_ui.admin
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- $eVal = variable_get( 'eVal', '' );
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- $qRange = variable_get( 'qRange', '' );
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-
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- /* check values in post query */
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-
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- if ( !isset( $data[ query_type ] ) )
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- {
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- return services_error( 'Missing blast attribute $query_type [ set necleotide or protein in post request of query_type ]', 406 );
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- }
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- if ( !isset( $data[ db_type ] ) )
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- {
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- return services_error( 'Missing blast attribute $db_type [ set necleotide or protein in post request of db_type ]', 406 );
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- }
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-
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- $query_type = $data[ query_type ];
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- $db_type = $data[ db_type ];
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-
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- // check the user input in $data
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- blast_validation( $query_type, $db_type, $data );
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-
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-
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- if ( isset( $data[ $eVal ] ) )
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- {
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- $eVal = $data[ $eVal ];
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- }
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- if ( isset( $data[ $culling_limit ] ) )
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- {
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- $qRange = $data[ $culling_limit ];
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- }
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-
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- if ( $query_type == 'nucleotide' )
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- {
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- if ( $db_type == 'nucleotide' )
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- {
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- $blast_program = 'blastn';
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- // code not working for wordvalue = 7 ?
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- $options_database = get_blast_database_options( $data[ db_type ] );
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- $Databasekey = array_search( $data[ Select_Database ], $options_database );
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- $advanced_options = advanced_options( $blast_program, $eVal, $qRange, $data );
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-
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- }
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- elseif ( $db_type == 'protein' )
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- {
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- $blast_program = 'blastx';
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- // how to get gap cost - question
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- $options_database = get_blast_database_options( $data[ db_type ] );
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- $Databasekey = array_search( $data[ Select_Database ], $options_database );
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- $advanced_options = advanced_options( $blast_program, $eVal, $qRange, $data );
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- }
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- }
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- elseif ( $query_type == 'protein' )
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- {
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- if ( $db_type == 'nucleotide' )
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- {
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- $blast_program = 'tblastn';
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- $options_database = get_blast_database_options( $data[ db_type ] );
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- $Databasekey = array_search( $data[ Select_Database ], $options_database );
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- $advanced_options = advanced_options( $blast_program, $eVal, $qRange, $data );
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-
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- }
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- elseif ( $db_type == 'protein' )
|
|
|
- {
|
|
|
- $blast_program = 'blastp';
|
|
|
- $options_database = get_blast_database_options( $data[ db_type ] );
|
|
|
- $Databasekey = array_search( $data[ Select_Database ], $options_database );
|
|
|
- $advanced_options = advanced_options( $blast_program, $eVal, $qRange, $data );
|
|
|
- }
|
|
|
- }
|
|
|
-
|
|
|
-
|
|
|
- return blast_ui_getblastJobId( $data[ query_type ], $data[ db_type ], $data[ seqQuery ], $Databasekey, $advanced_options );
|
|
|
-
|
|
|
-
|
|
|
- }
|
|
|
-/*
|
|
|
- ** Define the advanced Options for Blast
|
|
|
- ** @return : advanced_options according to blast_program
|
|
|
- */
|
|
|
-function advanced_options( $blast_program, $eVal, $qRange, $data )
|
|
|
- {
|
|
|
-
|
|
|
- if ( $blast_program == 'blastn' )
|
|
|
- {
|
|
|
- $wordvalue = intval( $data[ Word_size ] );
|
|
|
- $numAlign = intval( $data[ Max_target_sequences ] );
|
|
|
-
|
|
|
- $Data_GapCost = _get_gap( $blast_program );
|
|
|
- $gapkey = array_search( $data[ Gap_Costs ], $Data_GapCost );
|
|
|
-
|
|
|
- $gap = _set_gap( $gapkey );
|
|
|
- $Data_Mismatch_Scores = _get_match_mismatch( $blast_program );
|
|
|
-
|
|
|
- $Mismatch_Scores_key = array_search( $data[ Match_Mismatch_Scores ], $Data_Mismatch_Scores );
|
|
|
- $m_m = _set_match_mismatch( $Mismatch_Scores_key );
|
|
|
-
|
|
|
- return array(
|
|
|
- 'max_target_seqs' => $numAlign,
|
|
|
- 'evalue' => $eVal,
|
|
|
- 'word_size' => $wordvalue,
|
|
|
- 'gapopen' => $gap[ 'gapOpen' ],
|
|
|
- 'gapextend' => $gap[ 'gapExtend' ],
|
|
|
- 'penalty' => $m_m[ 'penalty' ],
|
|
|
- 'reward' => $m_m[ 'reward' ],
|
|
|
- 'culling_limit' => $qRange
|
|
|
- );
|
|
|
-
|
|
|
- }
|
|
|
- elseif ( $blast_program == 'blastx' )
|
|
|
- {
|
|
|
- $wordSize = intval( $data[ Word_size ] );
|
|
|
- $numAlign = intval( $data[ Max_target_sequences ] );
|
|
|
- $matrix = $data[ Matrix_options ];
|
|
|
- $gap = getGap( $matrix, 1 );
|
|
|
-
|
|
|
- return array(
|
|
|
- 'max_target_seqs' => $numAlign,
|
|
|
- 'evalue' => $eVal,
|
|
|
- 'word_size' => $wordSize,
|
|
|
- 'gapopen' => $gap[ 'gapOpen' ],
|
|
|
- 'gapextend' => $gap[ 'gapExtend' ],
|
|
|
- 'culling_limit' => $qRange,
|
|
|
- 'matrix' => $matrix
|
|
|
- );
|
|
|
- }
|
|
|
- elseif ( $blast_program == 'tblastn' )
|
|
|
- {
|
|
|
- $wordSize = intval( $data[ Word_size ] );
|
|
|
- $numAlign = intval( $data[ Max_target_sequences ] );
|
|
|
- $matrix = $data[ Matrix_options ];
|
|
|
- $gap = getGap( $matrix, 1 );
|
|
|
-
|
|
|
- return array(
|
|
|
- 'max_target_seqs' => $numAlign,
|
|
|
- 'evalue' => $eVal,
|
|
|
- 'word_size' => $wordSize,
|
|
|
- 'gapopen' => $gap[ 'gapOpen' ],
|
|
|
- 'gapextend' => $gap[ 'gapExtend' ],
|
|
|
- 'culling_limit' => $qRange,
|
|
|
- 'matrix' => $matrix
|
|
|
- );
|
|
|
- }
|
|
|
- elseif ( $blast_program == 'blastp' )
|
|
|
- {
|
|
|
- $wordSize = intval( $data[ Word_size ] );
|
|
|
- $numAlign = intval( $data[ Max_target_sequences ] );
|
|
|
- $matrix = $data[ Matrix_options ];
|
|
|
- $gap = getGap( $matrix, 1 );
|
|
|
-
|
|
|
- return array(
|
|
|
- 'max_target_seqs' => $numAlign,
|
|
|
- 'evalue' => $eVal,
|
|
|
- 'word_size' => $wordSize,
|
|
|
- 'gapopen' => $gap[ 'gapOpen' ],
|
|
|
- 'gapextend' => $gap[ 'gapExtend' ],
|
|
|
- 'culling_limit' => $qRange,
|
|
|
- 'matrix' => $matrix
|
|
|
- );
|
|
|
- }
|
|
|
- }
|
|
|
-
|
|
|
-
|
|
|
-/*
|
|
|
- ** Call the core API of Triple
|
|
|
- ** @return : Job_ID
|
|
|
- */
|
|
|
-
|
|
|
-function blast_ui_getblastJobId( $query_type, $db_type, $seqQuery, $Databasekey, $adv_options )
|
|
|
- {
|
|
|
- if ( $query_type == 'nucleotide' )
|
|
|
- {
|
|
|
- if ( $db_type == 'nucleotide' )
|
|
|
- {
|
|
|
- $blast_program = 'blastn';
|
|
|
- }
|
|
|
- elseif ( $db_type == 'protein' )
|
|
|
- {
|
|
|
- $blast_program = 'blastx';
|
|
|
- }
|
|
|
- }
|
|
|
- elseif ( $query_type == 'protein' )
|
|
|
- {
|
|
|
- if ( $db_type == 'nucleotide' )
|
|
|
- {
|
|
|
- $blast_program = 'tblastn';
|
|
|
- }
|
|
|
- elseif ( $db_type == 'protein' )
|
|
|
- {
|
|
|
- $blast_program = 'blastp';
|
|
|
- }
|
|
|
- }
|
|
|
-
|
|
|
- $query = variable_get('file_temporary_path', file_directory_temp()) . '/' . date( 'YMd_His' ) . '_query.fasta';
|
|
|
- file_put_contents( $query, $seqQuery );
|
|
|
- $blastdb_node = node_load( $Databasekey );
|
|
|
- $blastdb_name = $blastdb_node->db_name;
|
|
|
- $blastdb_with_path = $blastdb_node->db_path;
|
|
|
-
|
|
|
- // Now let each program process its own advanced options.
|
|
|
-
|
|
|
-
|
|
|
- $advanced_options = $adv_options;
|
|
|
- global $user;
|
|
|
-
|
|
|
- $output_filestub = date( 'YMd_His' );
|
|
|
- $job_args = array(
|
|
|
- 'program' => $blast_program,
|
|
|
- 'query' => $query,
|
|
|
- 'database' => $blastdb_with_path,
|
|
|
- 'output_filename' => $output_filestub,
|
|
|
- 'options' => $advanced_options
|
|
|
- );
|
|
|
-
|
|
|
- $job_id = tripal_add_job( t( 'BLAST (@program): @query', array(
|
|
|
- '@program' => $blast_program,
|
|
|
- '@query' => $query
|
|
|
- ) ), 'blast_job', 'run_BLAST_tripal_job', $job_args, $user->uid );
|
|
|
-
|
|
|
- ob_start();
|
|
|
- tripal_launch_job( 1, $job_id );
|
|
|
- ob_end_clean();
|
|
|
- return $job_id;
|
|
|
- // return getBlastData($job_id);
|
|
|
- }
|
|
|
-
|
|
|
-
|
|
|
-
|
|
|
-
|
|
|
-
|
|
|
-
|
|
|
-
|
|
|
-function getBlaststatus( $job_id )
|
|
|
- {
|
|
|
- /* $sql = "SELECT status FROM tripal_jobs WHERE job_id = :job_id ";
|
|
|
- $job_res = db_query($sql, array(':job_id' => $job_id))->fetchField();
|
|
|
- return $job_res; */
|
|
|
- }
|
|
|
-
|
|
|
-
|
|
|
-/*
|
|
|
- **Method returns file output data
|
|
|
- ** @param int $job_id
|
|
|
- ** web service url : http://localhost:8080/restapi/blast/job_id
|
|
|
- */
|
|
|
-
|
|
|
-function getBlastData( $job_id )
|
|
|
- {
|
|
|
- $result;
|
|
|
- $sql = "SELECT job_name FROM tripal_jobs WHERE job_id = :job_id ";
|
|
|
- $job_res = db_query( $sql, array(
|
|
|
- ':job_id' => $job_id
|
|
|
- ) )->fetchField();
|
|
|
-
|
|
|
- $file_path = str_replace( '_query.fasta', '.blast.asn', $job_res );
|
|
|
-
|
|
|
-
|
|
|
- if ( stristr( $file_path, "tblastn" ) )
|
|
|
- {
|
|
|
- $file_path = str_replace( 'BLAST (tblastn): /', '/', $file_path );
|
|
|
- }
|
|
|
- elseif ( stristr( $file_path, "blastx" ) )
|
|
|
- {
|
|
|
- $file_path = str_replace( 'BLAST (blastx): /', '/', $file_path );
|
|
|
- }
|
|
|
- elseif ( stristr( $file_path, "blastn" ) )
|
|
|
- {
|
|
|
- $file_path = str_replace( 'BLAST (blastn): /', '/', $file_path );
|
|
|
- }
|
|
|
- elseif ( stristr( $file_path, "blastp" ) )
|
|
|
- {
|
|
|
- $file_path = str_replace( 'BLAST (blastp): /', '/', $file_path );
|
|
|
- }
|
|
|
-
|
|
|
-
|
|
|
- if ( file_exists( $file_path ) )
|
|
|
- {
|
|
|
- $result = file_get_contents( $file_path );
|
|
|
- }
|
|
|
- else
|
|
|
- {
|
|
|
- $result = "The file $file_path does not exist";
|
|
|
- }
|
|
|
- return $result;
|
|
|
- }
|
|
|
-
|
|
|
-/*
|
|
|
- ** Used for tblastn
|
|
|
- */
|
|
|
-function getGap_cost()
|
|
|
- {
|
|
|
- return array(
|
|
|
- 'PAM30' => _get_gap_for_matrix( 'PAM30' ),
|
|
|
- 'PAM70' => _get_gap_for_matrix( 'PAM70' ),
|
|
|
- 'PAM250' => _get_gap_for_matrix( 'PAM250' ),
|
|
|
- 'BLOSUM80' => _get_gap_for_matrix( 'BLOSUM80' ),
|
|
|
- 'BLOSUM62' => _get_gap_for_matrix( 'BLOSUM62' ),
|
|
|
- 'BLOSUM45' => _get_gap_for_matrix( 'BLOSUM45' ),
|
|
|
- 'BLOSUM50' => _get_gap_for_matrix( 'BLOSUM50' ),
|
|
|
- 'BLOSUM90' => _get_gap_for_matrix( 'BLOSUM90' )
|
|
|
- );
|
|
|
- }
|