'My Amazing Link Type', 'process function' => 'mymodule_generate_linkout_mylinktype', ); return $types; } * @endcode * * 2) Implement the process function you specified to determine the URL * to be linked to depending on the blast hit. For a full description of * parameters available to your function, see tripal_blast_generate_linkout_link(). * @code function mymodule_generate_linkout_mylinktype($url_prefix, $hit, $info, $options = array()) { // Do some simple steps to generate the suffix based on the $hit. return l('url name', $url_prefix . $url_postfix); } * @endcode * * This module will automatically, * - Add your custom type to the "Link-out Type" select list on Blast Database * node add/edit forms. * - If your type is chosen by the user when the Blast Database is created, * then your process function will be used by blast_report.tpl.php to * determine the URL that should be used for each hit link. */ /** * Implements hook_blast_linkout_info(). * Provide information on basic link-out types: link, GBrowse, JBrowse. * * NOTE: Each item must have a 'name' and 'process function' to indicate the * human-readable name to be used in the Blast Database add/edit form and the * function to be used to determine the URL for each hit in BLAST results. */ function blast_ui_blast_linkout_info() { $types = array(); // A default link-out type requiring no information. $types['none'] = array( 'name' => 'None', 'process function' => 'tripal_blast_generate_linkout_none', 'help' => 'This will leave the blast results hits as plain text.', 'require_regex' => FALSE, 'require_db' => FALSE, ); $types['link'] = array( // Human-readable Type name to display to users in the BLAST Database // create/edit form. 'name' => 'Generic Link', // The function used to generate the URL to be linked to. // This function will have full access to the blast hit and database // prefix information and is expected to return a URL. 'process function' => 'tripal_blast_generate_linkout_link', // Help text to show in the BLAST Database create/edit form so that // users will know how to use this link-out type. Specifically, info // about your assumptions for the URL prefix are very helpful. // HTML is aloud but do not enclose in

. 'help' => 'The External Database choosen below provides its URL prefix when determining the URL to link-out to. If the link-out type is "Generic Link" then the hit identifier (determined using fasta header format or regular expression) is concatenated to the end of the url prefix. For example, if your hit is for "Chr01" and the URL prefix is "http://myfriendstripalsite.org/name/" then the complete URL is simply <a href="http://myfriendstripalsite.org/name/Chr01">Chr01</a>.', // Whether or not the link-out requires additional fields from the nodes. 'require_regex' => TRUE, 'require_db' => TRUE, ); $types['gbrowse'] = array( 'name' => 'GBrowse', 'process function' => 'tripal_blast_generate_linkout_gbrowse', 'help' => 'The link created will add a BLAST track to the GBrowse (specified by the External Database) that shows the HSPs as well as indicating the overall hit. It is assumed that the Reference of the GBrowse is the same as this BLAST database (even the names must be consistent). Furthermore, the URL prefix supplied is expected to have an empty query (?) or be properly ended (;). For example, "http://mydomain.com/gb/gbrowse/tripalus_databasica/?" OR "http://mydomain.com/gb/gbrowse/tripalus_databasica/?label=genes+markers;"', // Whether or not the link-out requires additional fields from the nodes. 'require_regex' => TRUE, 'require_db' => TRUE, ); $types['jbrowse'] = array( 'name' => 'JBrowse', 'process function' => 'tripal_blast_generate_linkout_jbrowse', 'help' => 'The link created will add a "Blast Result" track to the JBrowse (specified by the External Database) that shows the HSPs as well as indicating the overall hit. It is assumed that the Reference of the JBrowse is the same as this BLAST database (even the names must be consistent). Furthermore, the URL prefix supplied is expected to have an empty query (?) or be properly ended (&). For example, "http://mydomain.com/jbrowse/tripalus_databasica/?" OR "http://mydomain.com/jbrowse/tripalus_databasica/?tracks=genes,markers,blast&". Also the Blast Result track is NOT Displayed by default. Either include "blast" using the "tracks" directive in the URL prefix or specify it in your JBrowse.conf.', // Whether or not the link-out requires additional fields from the nodes. 'require_regex' => TRUE, 'require_db' => TRUE, ); return $types; } /** * Generate a basic link-out for a given hit. * * Essentially, concatenate the URL prefix with the extracted hit identifier * and return the URL to be displayed by the BLAST report template. * * @param $url_prefix * The URL prefix for the BLAST Database queried. * @param $hit * The blast XML hit object. This object has the following keys based on the * XML: Hit_num, Hit_id, Hit_def, Hit_accession, Hit_len and Hit_hsps. * Furthermore, a linkout_id key has beek added that contains the part of the * Hit_def extracted using a regex provided when the blastdb node was created. * @param $info * Additional information that may be useful in creating a link-out. Includes: * - query_name: the name of the query sequence. * - score: the score of the blast hit. * - e-value: the e-value of the blast hit. * @param $options * Any additional options needed to determine the type of link-out. None are * supported by this particular link-out type. * * @return * An html link. */ function tripal_blast_generate_linkout_link($url_prefix, $hit, $info, $options = array()) { if (isset($hit->{'linkout_id'})) { $hit_url = $url_prefix . $hit->{'linkout_id'}; // Split out the CGI params, if any $params = array(); if (!$paramstr=strstr($hit_url, '?')) { $url_prefix = $hit_url; } else { $url_parts = preg_split("/\?/", $hit_url); $url_prefix = $url_parts[0]; $param_list = preg_split("/\&/", $url_parts[1]); foreach ($param_list as $param) { $param_parts = preg_split("/=/", $param, 2); $params[$param_parts[0]] = $param_parts[1]; } }//URL contains CGI parameters return l( $hit->{'linkout_id'}, $url_prefix, array('attributes' => array('target' => '_blank'), 'query' => $params) ); } else { return FALSE; } } /** * Generate a GBrowse link-out with location information for a given hit. * * NOTE: Assumes the hit is a backbone feature in the GBrowse linked to. * Otherwise, the basic link can be used. * * @param $url_prefix * The URL prefix for the BLAST Database queried. * @param $hit * The blast XML hit object. This object has the following keys based on the * XML: Hit_num, Hit_id, Hit_def, Hit_accession, Hit_len and Hit_hsps. * Furthermore, a linkout_id key has beek added that contains the part of the * Hit_def extracted using a regex provided when the blastdb node was created. * @param $info * Additional information that may be useful in creating a link-out. Includes: * - query_name: the name of the query sequence. * - score: the score of the blast hit. * - e-value: the e-value of the blast hit. * @param $options * Any additional options needed to determine the type of link-out. None are * supported by this particular link-out type. * * @return * An html link. */ function tripal_blast_generate_linkout_gbrowse($url_prefix, $hit, $info, $options = array()) { // First we need to collect the HSPs to define the ranges we want to // display on the JBrowse. $ranges = array(); // We also keep track of all the coordinates in order to later // calculate the smallest and largest coordinate. $coords = array(); foreach($info['HSPs'] as $hsp) { $start = min($hsp['Hsp_hit-from'], $hsp['Hsp_hit-to']); $stop = max($hsp['Hsp_hit-from'], $hsp['Hsp_hit-to']); // Format the hsp for inclusion in the new track later. array_push($ranges, "$start..$stop"); // Add both the start & stop to the coordinate list. array_push($coords, $start, $stop); } // Calculate the minimum & maximum coordinates. $min = min($coords); $max = max($coords); // Now we are finally ready to build the URL. // First lets set the location of the hit so the GBrowse focuses in on the correct region. $query = array(); $query['ref'] = $hit->{'linkout_id'}; $query['start'] = $min; $query['stop'] = $max; // Next we want to add our BLAST hit to the GBrowse. $query['add'] = format_string( '!ref !trackname !featurename !hspcoords', array( '!ref' => $hit->{'linkout_id'}, '!trackname' => 'BLAST', '!featurename' => 'BlastHit', '!hspcoords' => join ("," , $ranges), ) ); // Highlight our newly added feature. $query['h_feat'] = 'BlastHit'; $hit_url = $url_prefix; //. $url_postfix; $url = l( $hit->{'linkout_id'}, $hit_url, array( 'query' => $query, 'attributes' => array('target' => '_blank') ) ); // For some reason GBrowse expects semi-colons (;&) to delineate query paramters // whereas Drupal throws ampherstands (&) in. This is to fix that. $url = str_replace('&',';&', $url); return $url; } /** * Generate a JBrowse link-out with location information for a given hit. * * NOTE: Assumes the hit is a backbone feature in the JBrowse linked to. * Otherwise, the basic link can be used. * NOTE: This linkout creates a "blast" track but doesn't make it visible. This is to * allow your default tracks to be visible and give contect to your blast hit. You * should include "blast" in your jbrowse.conf default track list to ensure your * users can always see their hits. If you don't have access to the jbrowse.conf, * you can place the tracks you want to see including 'blast' in the url prefix * (see example below under @param $url_prefix). * * @param $url_prefix * The URL prefix for the BLAST Database queried. It is assumed that the url prefix * includes the ? and if there are any key=vale pairs that the last symbol is &. * For example, * http://myserver.com/jbrowse/databasica/? * http://myserver.com/jbrowse/databasica/?tracks=myfavtrack,anoktrack,blast& * * @param $hit * The blast XML hit object. This object has the following keys based on the * XML: Hit_num, Hit_id, Hit_def, Hit_accession, Hit_len and Hit_hsps. * Furthermore, the following keys have been added: * -linkout_id: the part of the Hit_def extracted using a regex provided * when the blastdb node was created. * -hit_name: the name of the hit extracted in the template. * @param $info * Additional information that may be useful in creating a link-out. Includes: * - query_name: the name of the query sequence. * - score: the score of the blast hit. * - e-value: the e-value of the blast hit. * @param $options * Any additional options needed to determine the type of link-out. None are * supported by this particular link-out type. * * @return * An html link. */ function tripal_blast_generate_linkout_jbrowse($url_prefix, $hit, $info, $options = array()) { // First we need to collect the HSPs to define the ranges we want to // display on the JBrowse. $ranges = array(); // We also keep track of all the coordinates in order to later // calculate the smallest and largest coordinate. $coords = array(); $count = 0; $strands = array(); foreach($info['HSPs'] as $hsp) { $count++; $strand = '1'; $hsp_start = $hsp['Hsp_hit-from']; $hsp_end = $hsp['Hsp_hit-to']; $strands[] = $strand; // Handle alignments on the negative strand. if (($hsp_end - $hsp_start) < 0) { $strand = '-1'; $hsp_start = $hsp['Hsp_hit-to']; $hsp_end = $hsp['Hsp_hit-from']; } // Add both the start & stop to the coordinate list. array_push($coords,$hsp['Hsp_hit-from'] , $hsp['Hsp_hit-to'] ); // Format the hsp for inclusion in the subfeatures section of the track later. $hsp_def = format_string( '{"start":!start,"end":!end,"strand":"!strand","type":"!type"}', array( '!start' => $hsp_start, '!end' => $hsp_end, '!strand' => $strand, '!type' => 'match_part' ) ); array_push($ranges, $hsp_def); } // Calculate the minimum & maximum coordinates. $min = min($coords); $max = max($coords); // We also want some white-space on either side of out hit // when we show it in the JBrowse. To make this generic, // we want our blast hit to take up 2/3 of the screen thus // we have 1/6 per side for white-space. $buffer = round(($max - $min) / 6); $screen_start = $min - $buffer; $screen_end = $max + $buffer; // Now we are finally ready to build the URL. // First lets set the location of the hit so the JBrowse focuses in on the correct region. $jbrowse_query = array(); $jbrowse_query['loc'] = format_string( 'loc=!ref:!start..!stop', array( '!ref' => $hit->{'linkout_id'}, '!start' => $screen_start, '!stop' => $screen_end, ) ); $unique_strands = array_unique($strands); if (count($unique_strands) === 1) { $strand = end($strands); // Next we want to add our BLAST hit to the JBrowse. $jbrowse_query['addFeatures'] = format_string( 'addFeatures=[{"seq_id":"!id","start":!min,"end":!max,"name":"!name","strand":!strand,"subfeatures":[!hspcoords]}]', array( '!id' => $hit->{'linkout_id'}, '!name' => $info['query_name'] . ' Blast Hit', '!min' => $min, '!max' => $max, '!hspcoords' => join(",", $ranges), '!strand' => $strand, ) ); } else { $jbrowse_query['addFeatures'] = format_string( 'addFeatures=[{"seq_id":"!id","start":!min,"end":!max,"name":"!name","subfeatures":[!hspcoords]}]', array( '!id' => $hit->{'linkout_id'}, '!name' => $info['query_name'] . ' Blast Hit', '!min' => $min, '!max' => $max, '!hspcoords' => join(",", $ranges), ) ); } // Then add a track to display our new feature. $jbrowse_query['addTracks'] = 'addTracks=[{"label":"blast","key":"BLAST Result","type":"JBrowse/View/Track/CanvasFeatures","store":"url"}]'; $url_postfix = implode('&', $jbrowse_query); $hit_url = $url_prefix . $url_postfix; return l( $hit->{'linkout_id'}, $hit_url, array('attributes' => array('target' => '_blank')) ); }