'fieldset', '#title' => t('General parameters'), '#collapsible' => FALSE, ); $form['ALG']['GParam']['maxTarget'] = array( '#type' => 'select', '#title' => t('Max target sequences:'), '#options' => array( 0 => t('10'), 1 => t('50'), 2 => t('100'), 3 => t('250'), 4 => t('500'), 5 => t('1000'), 6 => t('5000'), 7 => t('10000'), 8 => t('20000'), ), '#default_value' => 2, '#description' => t('Select the maximum number of aligned sequences to display'), ); $form['ALG']['GParam']['shortQueries'] = array( '#type' => 'checkbox', '#title' => t('Automatically adjust parameters for short input sequences'), '#default_value' => TRUE, ); $form['ALG']['GParam']['eVal'] = array( '#type' => 'textfield', '#title' => t('e-Value (Expected Threshold)'), '#default_value' => 10, '#size' => 12, '#maxlength' => 20, '#description' => t('Expected number of chance matches in a random model. This number should be give in a decimal format. More Information | Expect value vedio tutorial'), ); $form['ALG']['GParam']['wordSize'] = array( '#type' => 'select', '#title' => t('Word size:'), '#options' => array( 0 => t('16'), 1 => t('20'), 2 => t('24'), 3 => t('28'), 4 => t('32'), 5 => t('48'), 6 => t('64'), 7 => t('128'), 8 => t('256'), ), '#default_value' => 3, '#description' => t('The length of the seed that initiates an alignment'), ); $form['ALG']['GParam']['qRange'] = array( '#type' => 'textfield', '#title' => t('Max matches in a query range'), '#default_value' => 0, '#size' => 12, '#maxlength' => 20, '#description' => t('Limit the number of matches to a query range. This option is useful if many strong matches to one part of a query may prevent BLAST from presenting weaker matches to another part of the query.'), ); // Scoring parameters //......................... $form['ALG']['SParam'] = array( '#type' => 'fieldset', '#title' => t('Scoring parameters'), '#collapsible' => FALSE, ); $form['ALG']['SParam']['M&MScores'] = array( '#type' => 'select', '#title' => t('Match/Mismatch Scores:'), '#options' => array( 0 => t('1,-2'), 1 => t('1,-3'), 2 => t('1,-4'), 3 => t('2,-3'), 4 => t('4,-5'), 5 => t('1,-1'), ), '#default_value' => 0, '#description' => t('Reward and penalty for matching and mismatching bases.'), ); $form['ALG']['SParam']['gapCost'] = array( '#type' => 'select', '#title' => t('Gap Costs:'), '#options' => array( 0 => t('Existence: 5 Extension: 2'), 1 => t('Existence: 2 Extension: 2'), 2 => t('Existence: 1 Extension: 2'), 3 => t('Existence: 0 Extension: 2'), 4 => t('Existence: 3 Extension: 1'), 5 => t('Existence: 2 Extension: 1'), 6 => t('Existence: 1 Extension: 1'), ), '#default_value' => 0, '#description' => t('Cost to create and extend a gap in an alignment. Linear costs are available only with megablast and are determined by the match/mismatch scores.'), ); } /** * Validate the advanced options provided by the BLASTn form above. * * @see blast_ui_blastn_advanced_options_form(). */ function blast_ui_blastn_advanced_options_form_validate($form, $form_state) { } /** * Processed the advanced options provided by the BLASTn form above. * * @see blast_ui_blastn_advanced_options_form(). */ function blast_ui_blastn_advanced_options_form_submit($form, $form_state) { $eVal = $form_state['values']['eVal']; $trgtKey = $form_state['values']['maxTarget']; $numAlign = $form['ALG']['GParam']['maxTarget']['#options'][$trgtKey]; $wsKey = $form_state['values']['wordSize']; $wordSize = $form['ALG']['GParam']['wordSize']['#options'][$wsKey]; // Expand Gap Cost key into open and extend penalties $gapKey = $form_state['values']['gapCost']; switch ($gapKey) { case 0: $gapOpen = 5; $gapExtend = 2; break; case 1: $gapOpen = 2; $gapExtend = 2; break; case 2: $gapOpen = 1; $gapExtend = 2; break; case 3: $gapOpen = 0; $gapExtend = 2; break; case 4: $gapOpen = 3; $gapExtend = 1; break; case 5: $gapOpen = 2; $gapExtend = 1; break; case 6: $gapOpen = 1; $gapExtend = 1; break; } // Epand Match/Mismatch option into penalty/reward values // @todo Amir: Is the switch supposed to be for $scoreKey? $scoreKey = $form_state['values']['M&MScores']; switch ($gapKey) { case 0: $penalty = -2; $reward = 1; break; case 1: $penalty = -3; $reward = 1; break; case 2: $penalty = -4; $reward = 1; break; case 3: $penalty = -3; $reward = 2; break; case 4: $penalty = -5; $reward = 4; break; case 5: $penalty = -1; $reward = 1; break; } return array( 'evalue' => $eVal, 'word_size' => $wordSize, 'gapopen' => $gapOpen, 'gapextend' => $gapExtend, 'penalty' => $penalty, 'reward' => $reward ); } /** * @section * BLASTx: Search protein database using a translated nucleotide query. * ---------------------------------------------------------------------------- */ /** * Adds the BLASTx Advanced Options to the passed in form. * * This form function is meant to be called within another form definition. * * @param $form * The form the advanced options should be added to. This form already * contains a $form['ALG'] fieldset meant to contain the advanced options. * @param $formstate * The current state fo the form passed in as $form. */ function blast_ui_blastx_advanced_options_form(&$form, $form_state) { $form['ALG']['GParam'] = array( '#type' => 'fieldset', '#title' => t('General parameters'), '#collapsible' => FALSE, ); $form['ALG']['GParam']['maxTarget'] = array( '#type' => 'select', '#title' => t('Max target sequences:'), '#options' => array( 0 => t('10'), 1 => t('50'), 2 => t('100'), 3 => t('250'), 4 => t('500'), 5 => t('1000'), 6 => t('5000'), 7 => t('10000'), 8 => t('20000'), ), '#default_value' => 2, '#description' => t('Select the maximum number of aligned sequences to display'), ); $form['ALG']['GParam']['eVal'] = array( '#type' => 'textfield', '#title' => t('e-Value (Expected Threshold)'), '#default_value' => 10, '#size' => 12, '#maxlength' => 20, '#description' => t('Expected number of chance matches in a random model. This number should be give in a decimal format. More Information | Expect value vedio tutorial'), ); } /** * Validate the advanced options provided by the BLASTn form above. * * @see blast_ui_blastx_advanced_options_form(). */ function blast_ui_blastx_advanced_options_form_validate($form, $form_state) { } /** * Processed the advanced options provided by the BLASTx form above. * * @see blast_ui_blastx_advanced_options_form(). */ function blast_ui_blastx_advanced_options_form_submit($form, $form_state) { $eVal = $form_state['values']['eVal']; $trgtKey = $form_state['values']['maxTarget']; $numAlign = $form['ALG']['GParam']['maxTarget']['#options'][$trgtKey]; return array( 'evalue' => $eVal, ); } /** * @section * BLASTp: Search protein database using a protein query. * ---------------------------------------------------------------------------- */ /** * Adds the BLASTp Advanced Options to the passed in form. * * This form function is meant to be called within another form definition. * * @param $form * The form the advanced options should be added to. This form already * contains a $form['ALG'] fieldset meant to contain the advanced options. * @param $formstate * The current state fo the form passed in as $form. */ function blast_ui_blastp_advanced_options_form(&$form, $form_state) { //General parameters $form['ALG']['GParam'] = array( '#type' => 'fieldset', '#title' => t('General parameters'), '#collapsible' => FALSE, ); $form['ALG']['GParam']['maxTarget'] = array( '#type' => 'select', '#title' => t('Max target sequences:'), '#options' => array( 0 => t('10'), 1 => t('50'), 2 => t('100'), 3 => t('250'), 4 => t('500'), 5 => t('1000'), 6 => t('5000'), 7 => t('10000'), 8 => t('20000'), ), '#default_value' => 2, '#description' => t('Select the maximum number of aligned sequences to display'), ); $form['ALG']['GParam']['shortQueries'] = array( '#type' => 'checkbox', '#title' => t('Automatically adjust parameters for short input sequences'), '#default_value' => TRUE, ); $form['ALG']['GParam']['eVal'] = array( '#type' => 'textfield', '#title' => t('e-value(Expect threshold)'), '#default_value' => 10, '#size' => 12, '#maxlength' => 20, '#description' => t('Expected number of chance matches in a random model.'), ); $form['ALG']['GParam']['wordSize'] = array( '#type' => 'select', '#title' => t('Word size:'), '#options' => array( 0 => t('2'), 1 => t('3'), ), '#default_value' => 1, '#description' => t('The length of the seed that initiates an alignment'), ); $form['ALG']['GParam']['qRange'] = array( '#type' => 'textfield', '#title' => t('Max matches in a query range'), '#default_value' => 0, '#size' => 12, '#maxlength' => 20, '#description' => t('Limit the number of matches to a query range. This option is useful if many strong matches to one part of a query may prevent BLAST from presenting weaker matches to another part of the query.'), ); // Scoring parameters $form['ALG']['SParam'] = array( '#type' => 'fieldset', '#title' => t('Scoring parameters'), '#collapsible' => FALSE, ); $options_first = _ajax_example_get_first_dropdown_options(); $selected = isset($form_state['values']['MATRIX'] ) ? $form_state['values']['MATRIX'] : key($options_first); $form['ALG']['SParam']['MATRIX'] = array( '#type' => 'select', '#title' => 'Matrix', '#options' => $options_first, '#default_value' => $selected, '#description' => t('Assigns a score for aligning pairs of residues, and determines overall alignment score..'), '#ajax' => array( 'callback' => 'ajax_example_dependent_dropdown_callback', 'wrapper' => 'dropdown-second-replace', ), ); $form['ALG']['SParam']['gapCost'] = array( '#type' => 'select', '#title' => t('Gap Costs:'), '#prefix' => '', '#options' => _ajax_example_get_second_dropdown_options($selected), '#default_value' => 2, '#description' => t('Cost to create and extend a gap in an alignment.'), ); $form['ALG']['SParam']['M&MScores'] = array( '#type' => 'select', '#title' => t('Match/Mismatch Scores:'), '#options' => array( 0 => t('No adjustment'), 1 => t('Composition-based statistics'), 2 => t('Conditional compositional score matrix adjustment'), 3 => t('Universal composition score matrix adjustment '), ), '#default_value' => 2, '#description' => t('Matrix adjustment method to compensate for amino acid composition of sequences'), ); } /** * Validate the advanced options provided by the BLASTn form above. * * @see blast_ui_blastp_advanced_options_form(). */ function blast_ui_blastp_advanced_options_form_validate($form, $form_state) { } /** * Processed the advanced options provided by the BLASTp form above. * * @see blast_ui_blastp_advanced_options_form(). */ function blast_ui_blastp_advanced_options_form_submit($form, $form_state) { $eVal = $form_state['values']['eVal']; $trgtKey = $form_state['values']['maxTarget']; $numAlign = $form['ALG']['GParam']['maxTarget']['#options'][$trgtKey]; $wsKey = $form_state['values']['wordSize']; $wordSize = $form['ALG']['GParam']['wordSize']['#options'][$wsKey]; // Expand Gap Cost key into open and extend penalties $gapKey = $form_state['values']['MATRIX']; switch ($gapKey) { case 0: $matrix ="PAM30"; $gapKey = $form_state['values']['gapCost']; switch ($gapKey) { case 0: $gapOpen = 7; $gapExtend = 2; break; case 1: $gapOpen = 6; $gapExtend = 2; break; case 2: $gapOpen = 5; $gapExtend = 2; break; case 3: $gapOpen = 10; $gapExtend = 1; break; case 4: $gapOpen = 9; $gapExtend = 1; break; case 5: $gapOpen = 8; $gapExtend = 1; break; } break; case 1: $matrix ="PAM70"; $gapKey = $form_state['values']['gapCost']; switch ($gapKey) { case 0: $gapOpen = 8; $gapExtend = 2; break; case 1: $gapOpen = 7; $gapExtend = 2; break; case 2: $gapOpen = 6; $gapExtend = 2; break; case 3: $gapOpen = 11; $gapExtend = 1; break; case 4: $gapOpen = 10; $gapExtend = 1; break; case 5: $gapOpen = 9; $gapExtend = 1; break; } break; case 2: $matrix ="PAM250"; $gapKey = $form_state['values']['gapCost']; switch ($gapKey) { case 0: $gapOpen = 15; $gapExtend = 3; break; case 1: $gapOpen = 14; $gapExtend = 3; break; case 2: $gapOpen = 13; $gapExtend = 3; break; case 3: $gapOpen = 12; $gapExtend = 3; break; case 4: $gapOpen = 11; $gapExtend = 3; break; case 5: $gapOpen = 17; $gapExtend = 2; break; case 6: $gapOpen = 16; $gapExtend = 2; break; case 7: $gapOpen = 15; $gapExtend = 2; break; case 8: $gapOpen = 14; $gapExtend = 2; break; case 9: $gapOpen = 13; $gapExtend = 2; break; case 10: $gapOpen = 21; $gapExtend = 1; break; case 11: $gapOpen = 20; $gapExtend = 1; break; case 12: $gapOpen = 19; $gapExtend = 1; break; case 13: $gapOpen = 18; $gapExtend = 1; break; case 14: $gapOpen = 17; $gapExtend = 1; break; } break; case 3: $matrix ="BLOSUM80"; $gapKey = $form_state['values']['gapCost']; switch ($gapKey) { case 0: $gapOpen = 8; $gapExtend = 2; break; case 1: $gapOpen = 7; $gapExtend = 2; break; case 2: $gapOpen = 6; $gapExtend = 2; break; case 3: $gapOpen = 11; $gapExtend = 1; break; case 4: $gapOpen = 10; $gapExtend = 1; break; case 5: $gapOpen = 9; $gapExtend = 1; break; } break; case 4: $matrix ="BLOSUM62"; $gapKey = $form_state['values']['gapCost']; switch ($gapKey) { case 0: $gapOpen = 11; $gapExtend = 2; break; case 1: $gapOpen = 10; $gapExtend = 2; break; case 2: $gapOpen = 9; $gapExtend = 2; break; case 3: $gapOpen = 8; $gapExtend = 2; break; case 4: $gapOpen = 7; $gapExtend = 2; break; case 5: $gapOpen = 6; $gapExtend = 2; break; case 6: $gapOpen = 13; $gapExtend = 1; break; case 7: $gapOpen = 12; $gapExtend = 1; break; case 8: $gapOpen = 11; $gapExtend = 1; break; case 9: $gapOpen = 10; $gapExtend = 1; break; case 10: $gapOpen = 9; $gapExtend = 1; break; } break; case 5: $matrix ="BLOSUM45"; $gapKey = $form_state['values']['gapCost']; switch ($gapKey) { case 0: $gapOpen = 13; $gapExtend = 3; break; case 1: $gapOpen = 12; $gapExtend = 3; break; case 2: $gapOpen = 11; $gapExtend = 3; break; case 3: $gapOpen = 10; $gapExtend = 3; break; case 4: $gapOpen = 15; $gapExtend = 2; break; case 5: $gapOpen = 14; $gapExtend = 2; break; case 6: $gapOpen = 13; $gapExtend = 2; break; case 7: $gapOpen = 12; $gapExtend = 2; break; case 8: $gapOpen = 19; $gapExtend = 1; break; case 9: $gapOpen = 18; $gapExtend = 1; break; case 10: $gapOpen = 17; $gapExtend = 1; break; case 11: $gapOpen = 16; $gapExtend = 1; break; } break; case 6: $matrix ="BLOSUM50"; $gapKey = $form_state['values']['gapCost']; switch ($gapKey) { case 0: $gapOpen = 13; $gapExtend = 3; break; case 1: $gapOpen = 12; $gapExtend = 3; break; case 2: $gapOpen = 11; $gapExtend = 3; break; case 3: $gapOpen = 10; $gapExtend = 3; break; case 4: $gapOpen = 9; $gapExtend = 3; break; case 5: $gapOpen = 16; $gapExtend = 2; break; case 6: $gapOpen = 15; $gapExtend = 2; break; case 7: $gapOpen = 14; $gapExtend = 2; break; case 8: $gapOpen = 13; $gapExtend = 2; break; case 9: $gapOpen = 12; $gapExtend = 2; break; case 10: $gapOpen = 19; $gapExtend = 1; break; case 11: $gapOpen = 18; $gapExtend = 1; break; case 12: $gapOpen = 17; $gapExtend = 1; break; case 13: $gapOpen = 16; $gapExtend = 1; break; case 14: $gapOpen = 15; $gapExtend = 1; break; } break; case 7: $matrix ="BLOSUM90"; $gapKey = $form_state['values']['gapCost']; switch ($gapKey) { case 0: $gapOpen = 9; $gapExtend = 2; break; case 1: $gapOpen = 8; $gapExtend = 2; break; case 2: $gapOpen = 7; $gapExtend = 2; break; case 3: $gapOpen = 6; $gapExtend = 2; break; case 4: $gapOpen = 11; $gapExtend = 1; break; case 5: $gapOpen = 10; $gapExtend = 1; break; case 6: $gapOpen = 9; $gapExtend = 1; break; } break; } return array( 'evalue' => $eVal, 'word_size' => $wordSize, 'gapopen' => $gapOpen, 'gapextend' => $gapExtend, 'matrix' => $matrix ); } /** * Fill the first dropdown list with appropriate options * * @return * An array consisting of matrices name for the first dropdown list */ function _ajax_example_get_first_dropdown_options() { return drupal_map_assoc(array( t('PAM30'), t('PAM70'), t('PAM250'), t('BLOSUM80'), t('BLOSUM62'), t('BLOSUM45'), t('BLOSUM50'), t('BLOSUM90'), )); } /** * Fill the second dropdown list with appropriate options * * @return * An array containing open and extension gap values for the chosen matrix (to fill the second dropdown list) */ function _ajax_example_get_second_dropdown_options($key = '') { $options = array( t('PAM30') => drupal_map_assoc(array( t('Existence: 7 Extension: 2'), t('Existence: 6 Extension: 2'), t('Existence: 5 Extension: 2'), t('Existence: 10 Extension: 1'), t('Existence: 9 Extension: 1'), t('Existence: 8 Extension: 1'), )), t('PAM70') => drupal_map_assoc(array( t('Existence: 8 Extension: 2'), t('Existence: 7 Extension: 2'), t('Existence: 6 Extension: 2'), t('Existence: 11 Extension: 1'), t('Existence: 10 Extension: 1'), t('Existence: 9 Extension: 1'), )), t('PAM250') => drupal_map_assoc(array( t('Existence: 15 Extension: 3'), t('Existence: 14 Extension: 3'), t('Existence: 13 Extension: 3'), t('Existence: 12 Extension: 3'), t('Existence: 11 Extension: 3'), t('Existence: 17 Extension: 2'), t('Existence: 16 Extension: 2'), t('Existence: 15 Extension: 2'), t('Existence: 14 Extension: 2'), t('Existence: 13 Extension: 2'), t('Existence: 21 Extension: 1'), t('Existence: 20 Extension: 1'), t('Existence: 19 Extension: 1'), t('Existence: 18 Extension: 1'), t('Existence: 17 Extension: 1'), )), t('BLOSUM80') => drupal_map_assoc(array( t('Existence: 8 Extension: 2'), t('Existence: 7 Extension: 2'), t('Existence: 6 Extension: 2'), t('Existence: 11 Extension: 1'), t('Existence: 10 Extension: 1'), t('Existence: 9 Extension: 1'), )), t('BLOSUM62') => drupal_map_assoc(array( t('Existence: 11 Extension: 2'), t('Existence: 10 Extension: 2'), t('Existence: 9 Extension: 2'), t('Existence: 8 Extension: 2'), t('Existence: 7 Extension: 2'), t('Existence: 6 Extension: 2'), t('Existence: 13 Extension: 1'), t('Existence: 12 Extension: 1'), t('Existence: 11 Extension: 1'), t('Existence: 10 Extension: 1'), t('Existence: 9 Extension: 1'), )), t('BLOSUM45') => drupal_map_assoc(array( t('Existence: 13 Extension: 3'), t('Existence: 12 Extension: 3'), t('Existence: 11 Extension: 3'), t('Existence: 10 Extension: 3'), t('Existence: 15 Extension: 2'), t('Existence: 14 Extension: 2'), t('Existence: 13 Extension: 2'), t('Existence: 12 Extension: 2'), t('Existence: 19 Extension: 1'), t('Existence: 18 Extension: 1'), t('Existence: 17 Extension: 1'), t('Existence: 16 Extension: 1'), )), t('BLOSUM50') => drupal_map_assoc(array( t('Existence: 13 Extension: 3'), t('Existence: 12 Extension: 3'), t('Existence: 11 Extension: 3'), t('Existence: 10 Extension: 3'), t('Existence: 9 Extension: 3'), t('Existence: 16 Extension: 2'), t('Existence: 15 Extension: 2'), t('Existence: 14 Extension: 2'), t('Existence: 13 Extension: 2'), t('Existence: 12 Extension: 2'), t('Existence: 19 Extension: 1'), t('Existence: 18 Extension: 1'), t('Existence: 17 Extension: 1'), t('Existence: 16 Extension: 1'), t('Existence: 15 Extension: 1'), )), t('BLOSUM90') => drupal_map_assoc(array( t('Existence: 9 Extension: 2'), t('Existence: 8 Extension: 2'), t('Existence: 7 Extension: 2'), t('Existence: 6 Extension: 2'), t('Existence: 11 Extension: 1'), t('Existence: 10 Extension: 1'), t('Existence: 9 Extension: 1'), )), ); if (isset($options[$key])) { return $options[$key]; } else { return array(); } } /** * Respond to Ajax dropdown call */ function ajax_example_dependent_dropdown_callback($form, $form_state) { return $form['ALG']['SParam']['gapCost']; } /** * @section * tBLASTn: Search translated nucleotide database using a protein query. * ---------------------------------------------------------------------------- */ /** * Adds the tBLASTn Advanced Options to the passed in form. * * This form function is meant to be called within another form definition. * * @param $form * The form the advanced options should be added to. This form already * contains a $form['ALG'] fieldset meant to contain the advanced options. * @param $formstate * The current state fo the form passed in as $form. */ function blast_ui_tblastn_advanced_options_form(&$form, $form_state) { $form['ALG']['GParam'] = array( '#type' => 'fieldset', '#title' => t('General parameters'), '#collapsible' => FALSE, ); $form['ALG']['GParam']['maxTarget'] = array( '#type' => 'select', '#title' => t('Max target sequences:'), '#options' => array( 0 => t('10'), 1 => t('50'), 2 => t('100'), 3 => t('250'), 4 => t('500'), 5 => t('1000'), 6 => t('5000'), 7 => t('10000'), 8 => t('20000'), ), '#default_value' => 2, '#description' => t('Select the maximum number of aligned sequences to display'), ); $form['ALG']['GParam']['eVal'] = array( '#type' => 'textfield', '#title' => t('e-Value (Expected Threshold)'), '#default_value' => 10, '#size' => 12, '#maxlength' => 20, '#description' => t('Expected number of chance matches in a random model. This number should be give in a decimal format. More Information | Expect value vedio tutorial'), ); } /** * Validate the advanced options provided by the BLASTn form above. * * @see blast_ui_tblastn_advanced_options_form(). */ function blast_ui_tblastn_advanced_options_form_validate($form, $form_state) { } /** * Processed the advanced options provided by the yBLASTn form above. * * @see blast_ui_tblastn_advanced_options_form(). */ function blast_ui_tblastn_advanced_options_form_submit($form, $form_state) { $eVal = $form_state['values']['eVal']; $trgtKey = $form_state['values']['maxTarget']; $numAlign = $form['ALG']['GParam']['maxTarget']['#options'][$trgtKey]; return array( 'evalue' => $eVal, ); }